Aptamer biosensor for protein detection based on guanine-quenching
Aptamer biosensor for protein detection based on guanine-quenching
复制标题
基于鸟嘌呤猝灭的蛋白质检测适体生物传感器
DOI：
10.1016/j.snb.2007.07.125
复制
发表时间：
2008-01-29
影响因子：
8.4
通讯作者：
Zhao, Xin Sheng
中科院分区：
文献类型：
--
作者：
Wang, Wenjuan;Chen, Chunlai;Zhao, Xin Sheng
基于鸟嘌呤碱基和荧光团之间的荧光猝灭，开发了一种经济高效的蛋白质检测生物传感器。在该生物传感器中，在适配体和蛋白质之间的识别过程中，通过适配体构象变化引起的荧光变化来检测未标记的蛋白质。使用凝血酶结合适体和凝血酶作为模型系统，开发了两种设计策略，即发夹设计和双链体设计。此外，我们还研究了发夹设计中修饰、茎长度和猝灭鸟嘌呤碱基数量或双链体设计中互补序列长度对检测限的影响。我们的结果表明，为了优化检测限，必须实现适体与蛋白质的结合能力和鸟嘌呤猝灭适体的猝灭效率之间的平衡。 (c) 2007 Elsevier B.V. 保留所有权利。
Based on fluorescence quenching between guanine bases and fluorophore, a cost-effective biosensor for protein detection is developed. In this biosensor, the label-free protein is detected through variation in fluorescence induced by aptamer conformational change during the recognition between aptamer and protein. Using thrombin-binding aptamer and thrombin as a model system, two design strategies, which are named as hairpin design and duplex design, are developed. Moreover, we investigated how the detection limit is influenced by the modifications, the stem length, and the number of quenching guanine bases in the hairpin design, or the length of the complementary sequence in the duplex design. Our results showed that, to optimize the detection limit, the balance between binding ability of aptamer to protein and quenching efficiency of guanine quenching aptamer have to be achieved. (c) 2007 Elsevier B.V. All rights reserved.