The bending rigidity of phospholipid monolayers in presence of an antimicrobial frog peptide studied by X-ray grazing incidence diffraction
The bending rigidity of phospholipid monolayers in presence of an antimicrobial frog peptide studied by X-ray grazing incidence diffraction
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DOI：
10.1016/j.physb.2004.11.053
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发表时间：
2005-02-28
影响因子：
2.8
通讯作者：
Lohner, K
中科院分区：
文献类型：
--
作者：
Konovalov, O;O'Flaherty, SM;Lohner, K
生物体的肽分泌构成了天然免疫系统所利用的整体反应过程。在这项工作中，我们提出了一个模型研究和洞察这个过程中报告的热力学和结构的影响，由于肽插入到磷脂单层。同步辐射X射线辐射与朗缪尔技术相结合，并利用形成“脂质肽”单分子膜和探测“肽分泌”的基本生物过程的物理特性。我们的实验表明，在磷脂层中插入肽对通过弯曲刚度表现的层的弹性性质具有不利影响。(C)2004 Elsevier B.V.保留所有权利。
Peptide secretion by living organisms constitutes an integral response process exploited by natural immune systems. In this work we present a model study and insight into this process reporting the thermodynamic and structural effects induced in phospholipid monolayers due to peptide insertion into the layer. Synchrotron X-ray radiation is combined with the Langmuir technique and exploited to form 'lipid-peptide' monolayers and probe the physical characteristics of the fundamental biological process of 'peptide secretion'. Our experiments show that the insertion of peptides in the phospholipid layer has adverse effects on the elastic properties of the layer manifested through the bending rigidity. (C) 2004 Elsevier B.V. All rights reserved.