Body Composition, Food Intake, and Energy Expenditure in a Murine Model of Roux-en-Y Gastric Bypass Surgery.
Body Composition, Food Intake, and Energy Expenditure in a Murine Model of Roux-en-Y Gastric Bypass Surgery.
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DOI：
10.1007/s11695-016-2062-y
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发表时间：
2016-09
期刊：
影响因子：
2.9
通讯作者：
Berthoud HR
中科院分区：
文献类型：
--
作者：
Hao Z;Mumphrey MB;Townsend RL;Morrison CD;Münzberg H;Ye J;Berthoud HR
Roux-en-Y胃旁路手术（RYGB）如此有效地降低体重和改善血糖控制的机制尚不清楚，小鼠模型对于确定所涉及的关键信号通路至关重要。表征RYGB对饮食性肥胖小鼠体重、体组成、食物摄入和能量消耗的时间过程，建立组织库，用于全球“组学”或靶向生化和结构分析。高脂肪饮食诱导的肥胖小鼠使用改进的手术技术与小胃袋进行RYGB。将对体重、身体组成、食物摄入和能量消耗的影响与假手术、高脂肪饮食限制体重匹配的对照组和从未肥胖的喂食对照组进行比较。在没有死亡和并发症的情况下，高脂肪饮食诱导的肥胖小鼠接受RYGB手术后，体重逐渐下降至平台期，并或多或少持续长达12周（33 g, - 18%, p<0.01），显著低于假手术小鼠（51 g, +25%, p<0.01），但高于年龄匹配的正常对照组小鼠（27 g） （+18%, p<0.01）。RYGB后的能量摄入仅在前10天与假手术相比明显受到抑制，但与体重匹配的小鼠相比明显更高。RYGB后的能量消耗在整个研究过程中都高于体重匹配的动物，但与假动物相比没有。在饮食诱导的肥胖小鼠中进行RYGB手术导致与人类相似的体重和身体组成变化，但与人类不同的是，这主要是通过增加能量消耗而不是减少食物摄入来实现的。
The mechanisms by which Roux-en-Y gastric bypass surgery (RYGB) so effectively lowers body weight and improves glycemic control are not well understood and murine models are essential for identifying the crucial signaling pathways involved. To characterize the time course of RYGB on body weight, body composition, food intake, and energy expenditure in diet-induced obese mice, and establish a tissue bank for global “omics” or targeted biochemical and structural analyses. High-fat diet-induced obese mice were subjected to RYGB using an improved surgical technique with a small gastric pouch. The effects on body weight, body composition, food intake, and energy expenditure were compared to sham surgery, high-fat diet-restricted weight-matched controls, and never obese chow-fed controls. Without mortality or complications, RYGB surgery in high-fat diet-induced obese mice gradually decreased body weight to a plateau that was more or less sustained for up to 12 weeks (33 g, −18%, p<0.01) and significantly lower compared with sham-operated mice (51 g, +25%, p <0.01), but higher (+18%, p<0.01) than age-matched, chow-fed control mice (27 g). Energy intake after RYGB was significantly suppressed compared to sham only for the first 10 days, but significantly higher compared to weight-matched mice. Energy expenditure after RYGB was higher throughout the study compared with weight-matched, but not sham animals. RYGB surgery in diet-induced obese mice results in similar body weight and body composition changes as observed in humans, but in contrast to humans, this is achieved mainly through increased energy expenditure rather than decreased food intake.