GLUCOSE-UPTAKE IN HUMAN ADIPOSE-TISSUE
GLUCOSE-UPTAKE IN HUMAN ADIPOSE-TISSUE
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DOI：
10.1016/0026-0495(87)90242-3
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发表时间：
1987-12-01
影响因子：
9.8
通讯作者：
BJORNTORP, P
中科院分区：
文献类型：
--
作者：
MARIN, P;REBUFFESCRIVE, M;BJORNTORP, P
口服100克葡萄糖和50微米Ci U-14C-葡萄糖给17名身体脂肪含量差异很大的女性。四小时后，通过腹部和股骨区域的针刺活检获得的脂肪组织中的放射性和体外葡萄糖代谢被测量。然后在给予葡萄糖后1天、1周和每月至7个月的重复活检中测量甘油三酯中的放射性。肥胖女性腹部脂肪细胞4小时后甘油三酯的葡萄糖标记高于股骨脂肪细胞。在接下来的一周内略有增加，然后呈指数下降，腹部的半衰期为12个月，股骨的半衰期为19个月。从测量的甘油三酯标记和身体脂肪质量的测定中估计葡萄糖碳在全身脂肪中的摄取量，发现其在给定葡萄糖的数量级上不到4%。体外研究表明，脂肪组织中吸收的大部分葡萄糖被转化为乳酸。如果这是在体内的情况，那么脂肪组织中的葡萄糖摄取很可能对体内总葡萄糖的动态平衡具有重要意义，特别是在肥胖者中，最多可能达到口服葡萄糖的三分之一到一半。然而，这些葡萄糖摄取的大部分会再次以乳酸的形式离开脂肪组织。标记在腹部脂肪细胞中的半衰期较短，这与这些脂肪细胞在体外脂肪分解增加的发现是一致的。
One hundred grams of glucose with 50 .mu.Ci U-14C-glucose were given orally to 17 women with widely varying amounts of body fat. Radioactivity and glucose metabolism in vitro were then measured in adipose tissue obtained by needle biopsies in the abdominal and femoral regions after four hours. Radioactivity in triglycerides was then measured in repeated biopsies 1 day, 1 week, and monthly up to 7 months after glucose administration. Glucose label in triglycerides after four hours was higher in abdominal than femoral adipocytes in obese women. It increased slightly during the following week, and then decreased exponentially with a half-life of 12 months in the abdominal region and 19 months in the femoral region. Uptake of glucose carbon in total body fat was estimated from the triglyceride label measured and determinations of body fat mass, and found to be in the order of less than 4% of given glucose. The studies in vitro suggested that much of the glucose taken up in adipose tissue is converted to lactate. If this is the case in vivo, then glucose uptake in adipose tissue might well be of significance for total body glucose homeostasis, particularly in obese subjects, amounting to maximally perhaps one third to one half of the oral glucose given. The majority of this glucose uptake would then, however, leave adipose tissue again as lactate. The shorter half-life of a label in abdominal adipocytes is in agreement with findings of increased lipolysis in these adipocytes in vitro.