In vivo gene transfer into the periventricular region by electroporation.
In vivo gene transfer into the periventricular region by electroporation.
复制标题
通过电穿孔将体内基因转移到脑室周围区域。
DOI：
10.2176/nmc.40.618
复制
发表时间：
2000
影响因子：
1.9
通讯作者：
N. Tamaki
中科院分区：
文献类型：
--
作者：
T. Kondoh;Y. Motooka;A. Bhattacharjee;T. Kokunai;N. Saito;N. Tamaki
在正常大鼠脑中尝试了电穿孔基因转移。将报告基因pEGFP-C1(25微克/5微升)注入幼年大鼠纹状体，用植入纹状体内的一对电极施加不同的方波电脉冲。5天后取出脑组织切片。组织学检查显示，高能量脉冲可引起广泛的组织损伤，而低能量脉冲(200-400mJ)可导致每个脑内300多个细胞被转移，这些细胞广泛分布于侧脑室管膜下区，并向纹状体延伸长突起。
Gene transfer by electroporation was attempted in the normal rat brain. The reporter gene pEGFP-C1 (25 micrograms/5 microliters) was injected into the striatum of young adult rats and various square electrical impulses were applied using a pair of electrodes implanted in the striatum. The brains were removed and sliced after 5 days. Histological examination revealed that the high energy impulses caused extensive tissue damage whereas lower energy impulses (200-400 mJ) resulted in the transfection of more than 300 cells per brain, which were widely distributed in the subependymal region of the lateral ventricle and extended long processes into the striatum.
Yuji Oshima：“通过电脉冲将目标基因转移到体内角膜内皮。”
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--