Strontium-substituted hydroxyapatite coatings synthesized by pulsed-laser deposition: In vitro osteoblast and osteoclast response
Strontium-substituted hydroxyapatite coatings synthesized by pulsed-laser deposition: In vitro osteoblast and osteoclast response
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DOI：
10.1016/j.actbio.2008.05.005
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发表时间：
2008-11-01
影响因子：
9.7
通讯作者：
Bigi, A.
中科院分区：
文献类型：
--
作者：
Capuccini, C.;Torricelli, P.;Bigi, A.
越来越多的证据表明锶对骨的有益作用，这证明了将锶掺入生物材料用于硬组织修复的兴趣越来越大。我们成功地合成了不同程度的锶替代钙（0、1、3和7at.%）的羟基磷灰石（HA）薄膜。通过脉冲激光沉积涂层显示出颗粒状表面和良好的结晶度，其随着锶含量的增加而略微减小。成骨细胞样MG 63细胞和人破骨细胞在薄膜上培养长达21天。MG 63细胞上生长的锶掺杂的HA涂层显示正常的形态，良好的增殖和增加的值的分化参数，而破骨细胞的数量是负面影响的存在下的锶。离子对骨细胞的积极影响在相对高锶含量（3-7%）的HA沉积涂层的情况下尤其明显，其中观察到碱性磷酸酶活性、骨钙素、I型胶原和骨保护素/TNF相关活化诱导的细胞因子受体比率的值显著增加，破骨细胞增殖的值显著降低。(C)2008 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The increasing interest in strontium incorporation into biomaterials for hard tissue repair is justified by the growing evidence of its beneficial effect on bone. We successfully synthesized hydroxyapatite (HA) thin films with different extents of strontium substitution for calcium (0, 1, 3 or 7 at.%) by pulsed-laser deposition. The coatings displayed a granular surface and a good degree of crystallinity, which slightly diminished as strontium content increased. Osteoblast-like MG63 cells and human osteoclasts were cultured on the thin films up to 21 days. MG63 cells grown on the strontium-doped HA coatings displayed normal morphology, good proliferation and increased values of the differentiation parameters, whereas the number of osteoclasts was negatively influenced by the presence of strontium. The positive effect of the ion on bone cells was particularly evident in the case of coatings deposited from HA at relatively high strontium contents (3-7%), where significantly increased values of alkaline phosphatase activity, osteocalcin, type I collagen and osteoprotegerin/TNF-related activation-induced cytokine receptor ratio, and considerably reduced values of osteoclast proliferation, were observed. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.