Two period homologs:: Circadian expression and photic regulation in the suprachiasmatic nuclei
Two period homologs:: Circadian expression and photic regulation in the suprachiasmatic nuclei
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DOI：
10.1016/s0896-6273(00)80417-1
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发表时间：
1997-12-01
期刊：
影响因子：
16.2
通讯作者：
Reppert, SM
中科院分区：
文献类型：
--
作者：
Shearman, LP;Zylka, MJ;Reppert, SM
我们已经鉴定了果蝇时期基因的哺乳动物同源物，并将其命名为PER？PERS蛋白与最近克隆和鉴定的另一种哺乳动物的蛋白(命名为PER1)有40%的氨基酸同源性。PER1和PER2蛋白与果蝇的每个蛋白都有几个同源性的区域，包括蛋白二聚化PAS结构域。系统发育分析支持哺乳动物Per基因家族的存在。在小鼠中，PER1和PER2的RNA水平在SCN和眼睛中表现出昼夜节律，这是生物钟的位置。SCN中的PER1和PER2 RNA均在主观夜间受光照射而增加，但在主观白天则不受影响。这些结果促进了我们对哺乳动物候选时钟元素的了解。
We have characterized a mammalian homolog of the Drosophila period gene and designated it Per?. The PERS protein shows >40% amino acid identity to the protein of another mammalian per homolog (designated Per1) that was recently cloned and characterized. Both PER1 and PER2 proteins share several regions of homology with the Drosophila PER protein, including the protein dimerization PAS domain. Phylogenetic analysis supports the existence of a family of mammalian per genes. In the mouse, Per1 and Per2 RNA levels exhibit circadian rhythms in the SCN and eyes, sites of circadian clocks. Both Per1 and Per2 RNAs in the SCN are increased by light exposure during subjective night but not during subjective day. The results advance our knowledge of candidate clock elements in mammals.