HEAT-SHOCK PROTEINS ARE DIFFERENTIALLY EXPRESSED IN HUMAN GASTROINTESTINAL CANCERS
HEAT-SHOCK PROTEINS ARE DIFFERENTIALLY EXPRESSED IN HUMAN GASTROINTESTINAL CANCERS
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DOI：
10.1016/s0960-7404(10)80036-2
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发表时间：
1995-08-01
影响因子：
2.3
通讯作者：
EVERS, BM
中科院分区：
文献类型：
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作者：
EHRENFRIED, JA;HERRON, BE;EVERS, BM
热休克蛋白（Hsp）是存在于所有物种中的应激反应基因; Hsp的增加可以赋予某些癌症的化疗抗性。本研究的目的是确定热休克蛋白在人胃癌，胰腺癌和结肠癌的表达。提取胃癌（n = 3）、胰腺癌（n = 6）和结肠癌（n = 8）的RNA和蛋白质，并进行北方和西方印迹。我们发现，热休克蛋白70和热休克蛋白27 mRNA水平的差异表达在胃肠道肿瘤; mRNA表达密切相关的蛋白水平，表明在转录水平的调节。此外，Hsp90和Rip蛋白在胃肠道肿瘤中呈组成性表达。我们的结论是热休克蛋白在人类中的差异表达，胃癌，胰腺癌和结肠癌，这些增加热休克蛋白发生组成性的，不是生理或环境压力的结果。癌细胞中热休克蛋白表达的增加可能会增强耐药性，并导致某些胃肠道癌对化疗药物的敏感性改变。
The heat shock proteins (Hsp) are stress-responsive genes present in all species; increases of Hsp can confer chemotherapeutic resistance to certain cancers. The purpose of this study was to determine Hsp expression in human gastric, pancreatic and colon cancers. Gastric (n = 3), pancreatic (n = 6) and colon (n = 8) cancers were extracted for RNA and protein, and Northern and Western blots performed. We found that hsp70 and hsp27 mRNA levels were differentially expressed in the gastrointestinal cancers; mRNA expression closely correlated with protein levels suggesting regulation at the level of transcription. In addition, Hsp90 and Rip proteins were constitutively expressed in the gastrointestinal cancers. We conclude that the Hsp are differentially expressed in human; gastric, pancreatic and colon cancers; these increases in Hsp occur constitutively and are not the result of physiological or environmental stresses. Increases of Hsp expression in cancer cells may enhance resistance and account for the altered sensitivity of certain gastrointestinal cancers to chemotherapeutic agents.