Oral Phosphatidylcholine Improves Intestinal Barrier Function in Drug-Induced Liver Injury in Rats
Oral Phosphatidylcholine Improves Intestinal Barrier Function in Drug-Induced Liver Injury in Rats
复制标题
口服磷脂酰胆碱改善药物性肝损伤大鼠的肠屏障功能
DOI：
10.1155/2019/8723460
复制
发表时间：
2019-09-02
影响因子：
2
通讯作者：
Pan, Hongying
中科院分区：
文献类型：
--
作者：
Chen, Meijuan;Huang, Haijun;Pan, Hongying
目标。磷脂酰胆碱（PC）是主要的表面活性磷脂，对表面产生疏水性。据报道，它可以逆转肝纤维化的进展并改善肝功能。本研究旨在评价口服PC对药物性肝损伤大鼠肠屏障功能（IBF）的影响。方法。四氯化碳（CCl4-）致肝损伤大鼠给予100 mg/kg PC， 1次/ d，连续治疗21 d。研究了PC治疗对(i)肝功能和门静脉压力、（ii）肠道和肝脏组织学、（iii）血浆内毒素、二胺氧化酶（DAO）和肿瘤坏死因子- (TNF-) α水平的影响。结果。PC治疗可降低ccl4所致肝损伤的门静脉压，改善肝功能。在pc处理的肝损伤大鼠中，胶原纤维逐渐减少，肝细胞排列紊乱和肝小叶紊乱得到部分修复，纤维组织中炎症细胞浸润减少。在pc治疗的肝硬化大鼠中，回肠炎症细胞浸润减少，肠道组织学改善，血清DAO水平降低。正如血清tnf - α水平和血浆内毒素水平降低所表明的那样，这些变化与炎症活性降低有关。结论。这些结果表明，PC治疗具有肝保护作用，能够恢复药物性肝损伤大鼠的IBF，减少内毒素血症。
Objective. Phosphatidylcholine (PC) is the major surface-active phospholipid and creates a hydrophobic nature to the surface. It has been reported to reverse the progression of liver fibrosis and to improve liver function. The aim of the present study was to evaluate the effects of orally administered PC on intestinal barrier function (IBF) in rats with drug-induced liver injury. Method. Rats with carbon tetrachloride- (CCl4-) induced liver injury were treated with 100 mg/kg PC once daily for 21 days. The effects of PC therapy on (i) liver function and portal pressure, (ii) intestinal and hepatic histology, and (iii) plasma endotoxin, diamine oxidase (DAO), and tumour necrosis factor- (TNF-) alpha levels were investigated. Results. PC therapy reduced portal pressure and improved the liver function in CCl4-induced liver injury. In PC-treated liver injury rats, collagen fibres were gradually decreased, while the disordered arrangement of hepatocytes and disorganized hepatic lobules were partially repaired, and inflammatory cell infiltration was decreased in the fibrous tissue. Lower inflammatory cell infiltration in the ileum improved intestinal histology, and reduced serum DAO levels were observed in PC-treated cirrhotic rats. These changes were associated with reduced inflammatory activity, as indicated by decreased serum TNF-alpha levels and plasma endotoxin levels. Conclusions. These results suggest that PC therapy is hepatoprotective and is able to restore IBF and reduce endotoxaemia in rats with drug-induced liver injury.