Comparative transcriptome analysis of resistant and susceptible kiwifruits in response to Pseudomonas syringae pv. Actinidiae during early infection
Comparative transcriptome analysis of resistant and susceptible kiwifruits in response to Pseudomonas syringae pv. Actinidiae during early infection
复制标题
抗性和易感猕猴桃对丁香假单胞菌的反应的比较转录组分析。
DOI：
10.1371/journal.pone.0211913
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发表时间：
2019-02-19
期刊：
影响因子：
3.7
通讯作者：
Fang, Jinbao
中科院分区：
文献类型：
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作者：
Song, Yalin;Sun, Leiming;Fang, Jinbao
猕猴桃细菌性溃疡病是一种威胁猕猴桃生产的毁灭性疾病。目的：阐明丁香假单胞菌的防御机制。在接种Psa后0、12、24、48、96和144小时，我们观察了抗性华特猕猴桃（HT）和敏感的红阳猕猴桃（HY）品种的表型变化。HY芽在接种区12 h出现棕色病变，病变长度从24 h逐渐增加到144 h，而HT芽在任何时间点均未见病变。此外，RNA-seq分析显示，在12岁时，HT和HY之间的差异表达基因明显多于其他任何时间点。通过加权基因共表达网络分析，HT与HY之间有5个模块的表达存在显著差异；利用京都基因与基因组百科数据库对这5个模块进行通路定位。在MEgreenyellow和MEyellow模块中，富集了与“植物-病原体相互作用”、“内吞噬”、“甘氨酸、丝氨酸和苏氨酸代谢”和“光合生物固碳”相关的途径，而在MEblack模块中，富集了与“内质网蛋白质加工”、“植物-病原体相互作用”和“糖酵解/糖异生”相关的途径。特别是编码效应受体的Pti1和RPS2，以及水杨酸信号通路相关的NPR1、TGA和PR1基因在HT中较HY显著上调，表明效应触发的免疫应答更强，水杨酸信号通路在HT的Psa防御应答中起着关键作用。此外，我们还发现了其他重要的基因，涉及苯丙素生物合成和Ca2+内部流动，这些基因在HT中高度表达。综上所述，这些结果为阐明猕猴桃在Psa感染过程中的防御机制提供了重要信息。
Kiwifruit bacterial canker is a devastating disease threatening kiwifruit production. To clarify the defense mechanism in response to Pseudomonas syringae pv. actinidiae (Psa), we observed phenotypic changes in resistant Huate (HT) and susceptible Hongyang (HY) kiwifruit varieties at 0, 12, 24, 48, 96, and 144 hour after inoculation (hai) with Psa. Brown lesions appeared in the inoculation areas 12 hai in HY shoots, and the lesion length gradually increased from 24 to 144 h. In contrast, no lesions were found in HT shoots at any time points. Furthermore, RNA-seq analysis showed significantly more differentially expressed genes between HT and HY at 12 hai than at any other time point. According to weighted gene co-expression network analysis, five modules were notably differentially expressed between HT and HY; pathway mapping using the Kyoto Encyclopedia of Gene and Genomes database was performed for the five modules. In MEgreenyellow and MEyellow modules, pathways related to“plant-pathogen interaction”, “Endocytosis”, “Glycine, serine and threonine metabolism”, and “Carbon fixation in photosynthetic organisms” were enriched, whereas in the MEblack module, pathways related to “protein processing in endoplasmic reticulum”, “plant-pathogen interaction”, and “Glycolysis / Gluconeogenesis” were enriched. In particular, the Pti1 and RPS2 encoding effector receptors, and the NPR1, TGA, and PR1 genes involved in the salicylic acid signaling pathway were significantly up-regulated in HT compared with HY. This indicates that the effector-triggered immunity response was stronger and that the salicylic acid signaling pathway played a pivotal role in the Psa defense response of HT. In addition, we identified other important genes, involved in phenylpropanoid biosynthesis and Ca2+ internal flow, which were highly expressed in HT. Taken together, these results provide important information to elucidate the defense mechanisms of kiwifruit during Psa infection.