A Salmonella-based, propionate-inducible, expression system for Salmonella enterica.
A Salmonella-based, propionate-inducible, expression system for Salmonella enterica.
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DOI：
10.1016/j.gene.2006.02.013
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发表时间：
2006-08
期刊：
影响因子：
3.5
通讯作者：
Sung Kuk Lee;J. Keasling
中科院分区：
文献类型：
--
作者：
Sung Kuk Lee;J. Keasling
The expression and regulatory properties of a propionate-regulated overexpression system (Salmonella enterica prpBCDE promoter (PprpB) and positive regulator (prpR)) were evaluated in wild-type S. enterica serovar Typhimurium TR6583 and prpB−or prpD−versions of this strain and compared with the arabinose-regulated T7 expression system. The wild-type strain showed low expression in the absence of propionate and high expression in the presence of propionate under all growth conditions. In 96-well plates and culture tubes, the wild-type strain exhibited a long delay before full induction; the time delay was significantly shorter in shake flasks. The prpD−strain exhibited low expression in the presence of glucose, highly regulatable expression over a wide range of propionate concentrations, and, in contrast to the wild-type strain, fast induction to full expression under all growth conditions. In contrast, the prpB−strain showed very high background expression in both culture tubes and shake flasks.
