Fission yeast switches mating type by a replication-recombination coupled process
Fission yeast switches mating type by a replication-recombination coupled process
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DOI：
10.1093/emboj/19.6.1389
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发表时间：
2000-03-15
期刊：
影响因子：
11.4
通讯作者：
de Lahondès, R
中科院分区：
文献类型：
--
作者：
Arcangioli, B;de Lahondès, R
裂变酵母表现出同宗配合的生命周期，其中细胞的交配类型以高度调节的方式在有丝分裂中交替。分裂细胞的谱系分析表明，两个姐妹细胞中只有一个转换交配类型。最近的研究表明，在mat 1位点的位点特异性和链特异性DNA修饰先于交配型转换。通过PCR方法追踪mat 1 DNA在细胞周期中的命运，我们发现了一种新的S期交配型转换的DNA中间体，这种DNA中间体的出现和消失的时间和速率与交配型转换通过复制-重组偶联途径发生的模型一致。这样的过程提供了实验证据，支持在粟酒裂殖酵母交配型转换的复制选择重组模型，并让人想起在某些类型的DNA损伤的存在下，用于完成复制的姐妹染色单体重组。
Fission yeast exhibits a homothallic life cycle, in which the mating type of the cell mitotically alternates in a highly regulated fashion. Pedigree analysis of dividing cells has shown that only one of the two sister cells switches mating type. It was shown recently that a site-and strand-specific DNA modification at the mat1 locus precedes mating-type switching. By tracking the fate of mat1 DNA throughout the cell cycle with a PCR assay, we identified a novel DNA intermediate of mating-type switching in S-phase, The time and rate of appearance and disappearance of this DNA intermediate are consistent with a model in which mating-type switching occurs through a replication-recombination coupled pathway. Such a process provides experimental evidence in support of a copy choice recombination model in Schizosaccharomyces pombe mating-type switching and is reminiscent of the sister chromatid recombination used to complete replication in the presence of certain types of DNA damage.