Genome duplication effects on pollen development and the interrelated physiological substances in tetraploid rice with polyploid meiosis stability
Genome duplication effects on pollen development and the interrelated physiological substances in tetraploid rice with polyploid meiosis stability
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多倍体减数分裂稳定性四倍体水稻基因组重复对花粉发育及相关生理物质的影响
DOI：
10.1007/s00425-010-1249-z
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发表时间：
2010-10-01
期刊：
影响因子：
4.3
通讯作者：
Cai, Detian
中科院分区：
文献类型：
--
作者：
He, Yuchi;Wei, Qiong;Cai, Detian
由于结实率低，多倍体水稻育种长期以来未能取得突破。多倍体减数分裂稳定性（PMeS）材料的发现和应用为解决这一问题发挥了关键作用。我们的结果表明，基因组复制导致不同水稻品种在花粉育性、活力以及游离脯氨酸和内源激素等重要生理物质的积累方面产生不同的结果。来自 PMeS HN2026-4X 品系的花粉表现出与 HN2026-2X 相似的高育性和活力（4X 表示四倍体，2X 表示二倍体），而在 Ballilla-4X 中，这两个比率均显着下降。花粉显微结构和超微结构研究结果表明，HN2026-4X的花粉发育模式在各个阶段都表现正常，但Balilla-4X却发现了很多变化。稳定的减数分裂、及时的绒毡层降解和正常的线粒体发育是保证PMeS水稻高频花粉育性的关键因素。与HN2026-2X相比，HN2026-4X中游离脯氨酸含量显着增加，但Balilla-4X中游离脯氨酸含量下降。基因组复制对调节花粉内源激素积累的影响很明显，导致PMeS HN2026-4X和Balilla-4X之间存在明显差异。成熟花粉中IAA、ZR和GA的积累将HN2026-4X与Balilla-4X区分开来，这与正常花粉发育有关。特别是，减数分裂阶段 ABA 的过度积累可能与 Ballilla-4X 减数分裂混乱有关。所有结果都提供了明确的证据，表明基因组复制在 PMeS HN2026-4X 的正常花粉发育中发挥了特定作用。
The breeding of polyploid rice made no breakthrough for a long time because of low seed set. The discovery and application of polyploid meiosis stability (PMeS) material played a pivotal role in solving this problem. Our results indicated that genome duplication led to different outcomes in different rice cultivars in terms of pollen fertility, viability, and the accumulation of important physiological substances such as free proline and endogenous hormones. Pollen from the PMeS HN2026-4X lines showed a high fertility and viability similar to those of HN2026-2X (4X indicates tetraploid while 2X indicates the diploid), whereas both rates decreased dramatically in Balilla-4X. The results of pollen microstructure and ultrastructure investigations suggested that the pollen development pattern in HN2026-4X appeared normal at all stages, but a lot of changes were discovered in Balilla-4X. Stable meiosis, timely tapetum degradation, and normal mitochondria development were critical factors insuring the high frequency pollen fertility of PMeS rice. The free proline content increased markedly in HN2026-4X as compared to HN2026-2X, but it was decreased for Balilla-4X. Genome duplication effects on regulating endogenous hormones accumulation in pollen were evident, resulting in the clear difference between PMeS HN2026-4X and Balilla-4X. The accumulation of IAA, ZR, and GA in mature pollen distinguished HN2026-4X from Balilla-4X, which was linked to normal pollen development. In particular, the excessive accumulation of ABA at the meiosis stage may be correlated to disorganized meiosis in Balilla-4X. All the results provided unequivocal evidence that genome duplication played specific roles in the normal pollen development of PMeS HN2026-4X.