Effect of cyclosporin A on functional recovery in the spinal cord following contusion injury
Effect of cyclosporin A on functional recovery in the spinal cord following contusion injury
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DOI：
10.1111/j.1469-7580.2009.01107.x
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发表时间：
2009-09-01
影响因子：
2.4
通讯作者：
Barry, Frank P.
中科院分区：
文献类型：
--
作者：
McMahon, Siobhan S.;Albermann, Silke;Barry, Frank P.
大量证据表明，免疫抑制剂环孢素A（CsA）可能具有神经保护特性，可用于治疗脊髓损伤。本研究的目的是研究脊髓损伤后的细胞环境，并确定CsA是否对改变细胞相互作用以促进生长环境有影响。CsA给药一组大鼠4天后，他们忍受了中度挫伤。在损伤后3、5和7周使用Basso Beattie Bresnahan（BBB）运动评定量表评估功能恢复。伤后3周和7周处死大鼠，脊髓切片，用组织学和免疫组织化学方法染色并分析。使用体视学，检查CsA治疗组和对照组的病变大小和细胞环境。两组之间观察到的病变体积差异不大。在CsA治疗的动物中，在3周时观察到功能恢复的改善。虽然我们没有看到组织损伤的显着减少，但导致病变的单个细胞的比例存在一些显着差异。CsA给药可用作一种控制病变细胞群的技术，一旦确定了再生的理想环境和损伤后不同时间点CsA给药的效果，则使其更允许神经元再生。
Considerable evidence has shown that the immunosuppressant drug cyclosporin A (CsA) may have neuroprotective properties which can be exploited in the treatment of spinal cord injury. The aim of this study was to investigate the cellular environment within the spinal cord following injury and determine whether CsA has an effect on altering cellular interactions to promote a growth-permissive environment. CsA was administered to a group of rats 4 days after they endured a moderate contusion injury. Functional recovery was assessed using the Basso Beattie Bresnahan (BBB) locomotor rating scale at 3, 5 and 7 weeks post-injury. The rats were sacrificed 3 and 7 weeks post-injury and the spinal cords were sectioned, stained using histological and immunohistochemical methods and analysed. Using stereology, the lesion size and cellular environment in the CsA-treated and control groups was examined. Little difference in lesion volume was observed between the two groups. An improvement in functional recovery was observed within CsA-treated animals at 3 weeks. Although we did not see significant reduction in tissue damage, there were some notable differences in the proportion of individual cells contributing to the lesion. CsA administration may be used as a technique to control the cell population of the lesion, making it more permissive to neuronal regeneration once the ideal environment for regeneration and the effects of CsA administration at different time points post-injury have been identified.