The impact of temperature on marine phytoplankton resource allocation and metabolism
The impact of temperature on marine phytoplankton resource allocation and metabolism
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DOI：
10.1038/nclimate1989
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发表时间：
2013-11-01
影响因子：
30.7
通讯作者：
Mock, T.
中科院分区：
文献类型：
--
作者：
Toseland, A.;Daines, S. J.;Mock, T.
海洋浮游植物负责全球每年固定的二氧化碳的50%，并对海洋中的其他生物地球化学循环做出巨大贡献（1）。它们的贡献在很大程度上取决于动态环境条件与代谢反应之间的相互作用，而代谢反应是资源分配的基础，因此也是海洋生物地球化学循环的基础。然而，这些复杂的环境-生物群落相互作用尚未在更大范围内进行研究。在这里，我们使用了一套综合的方法，结合联合收割机元转录组，生化数据，细胞生理学和新兴的浮游植物生长策略在全球生态系统模型，表明温度显着影响真核浮游植物的代谢与全球变暖下的生态地球化学循环的后果。特别是，蛋白质合成的速率在高温下强烈增加，即使核糖体及其相关的rRNA的数量减少。因此，在较高的温度下，真核浮游植物似乎需要较低密度的核糖体来产生所需数量的细胞蛋白质。在温暖的海洋中，富含磷酸盐的核糖体（2）的减少往往会产生更高的有机氮（N）与磷酸盐（P）的比例，从而增加对N的需求，并由于向N限制的转变而对海洋碳循环产生影响。我们的综合方法表明，温度在资源分配和海洋浮游植物化学计量中起着以前未被认识到的关键作用，并对它们所驱动的海洋地球化学循环产生影响。
Marine phytoplankton are responsible for similar to 50% of the CO2 that is fixed annually worldwide, and contribute massively to other biogeochemical cycles in the oceans(1). Their contribution depends significantly on the interplay between dynamic environmental conditions and the metabolic responses that underpin resource allocation and hence biogeochemical cycling in the oceans. However, these complex environment-biome interactions have not been studied on a larger scale. Here we use a set of integrative approaches that combine metatranscriptomes, biochemical data, cellular physiology and emergent phytoplankton growth strategies in a global ecosystems model, to show that temperature significantly affects eukaryotic phytoplankton metabolism with consequences for biogeochemical cycling under global warming. In particular, the rate of protein synthesis strongly increases under high temperatures even though the numbers of ribosomes and their associated rRNAs decreases. Thus, at higher temperatures, eukaryotic phytoplankton seem to require a lower density of ribosomes to produce the required amounts of cellular protein. The reduction of phosphate-rich ribosomes(2) in warmer oceans will tend to produce higher organismal nitrogen (N) to phosphate (P) ratios, in turn increasing demand for N with consequences for the marine carbon cycle due to shifts towards N-limitation. Our integrative approach suggests that temperature plays a previously unrecognized, critical role in resource allocation and marine phytoplankton stoichiometry, with implications for the biogeochemical cycles that they drive.