NUCLEOTIDE-SEQUENCE OF A GENOMIC AND A CDNA CLONE ENCODING AN EXTRACELLULAR ALKALINE PROTEASE OF ASPERGILLUS-FUMIGATUS
NUCLEOTIDE-SEQUENCE OF A GENOMIC AND A CDNA CLONE ENCODING AN EXTRACELLULAR ALKALINE PROTEASE OF ASPERGILLUS-FUMIGATUS
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DOI：
10.1111/j.1574-6968.1992.tb05253.x
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发表时间：
1992-04-15
影响因子：
2.1
通讯作者：
MONOD, M
中科院分区：
文献类型：
--
作者：
JATONOGAY, K;SUTER, M;MONOD, M
烟曲霉胞外碱性蛋白酶(ALP)是枯草杆菌毒素家族的一种酶，是该真菌潜在的毒力因子。编码碱性磷酸酶的基因是从一株烟曲霉菌的基因组文库中分离出来的。将该基因的核苷酸序列与米曲霉ALP编码基因和烟曲霉菌ALP成熟蛋白编码基因进行了序列比较。成熟烟曲霉菌碱性磷酸酶含282个氨基酸，由3个外显子编码。前酶原具有121个氨基酸的前导序列。
An Aspergillus fumigatus extracellular alkaline protease (ALP) which is an enzyme of the subtilisin family is a potential virulent factor of the fungus. The gene encoding ALP was isolated from a genomic library made from DNA of an A. fumigatus isolate. The nucleotide sequence of this gene was compared to that of a cDNA encoding A. oryzae ALP and to that of a cDNA from A. fumigatus encoding the mature ALP protein. Mature A. fumigatus ALP contains 282 amino acids and is encoded by three exons. The pre-proenzyme has a leader sequence of 121 amino acids.