High-yield cultivation of Botryococcus braunii for biomass and hydrocarbons
High-yield cultivation of Botryococcus braunii for biomass and hydrocarbons
复制标题
高产栽培布氏葡萄球菌以获取生物质和碳氢化合物
DOI：
10.1016/j.biombioe.2019.105399
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发表时间：
2019
影响因子：
6
通讯作者：
Yuanguang Li
中科院分区：
文献类型：
--
作者：
Minxi Wan;Zhen Zhang;Ruixuan Wang;Wenmin Bai;Jianke Huang;Weiliang Wang;Guomin Shen;Anquan Yu;Yuanguang Li
布朗葡萄藻是一种高碳氢化合物含量的能源微藻。光合自养栽培是必不可少的，但生产力因长时间的种子准备而降低。因此，提出了一种利用异养种子进行光合自养培养的新策略。采用恒pH补料分批异养培养，在5L发酵罐中培养28 d，菌体生物量达到37.02g L-1。验证了异养种子接种在光合自养培养中的效果。在室内1 L鼓泡塔光生物反应器中，烃类的最终含量和产率分别为35.9%和128.3 mg L−1d− 1，为迄今为止报道的最高值。在室外3 L平板光生物反应器和120 L开环池中进行光合自养培养，最终烃类含量和产率分别为27.85%和35.06 mg L−1d−1，为利用B.布劳尼。
Botryococcus braunii is one of the most promising energy microalgae with a high content of hydrocarbons. Photoautotrophic cultivation is essential while the productivity is lowered by the long period of preparation for seeds. Therefore, a new strategy of photoautotrophic culture with heterotrophic seeds was proposed. The pH-auxostat fed-batch heterotrophic cultivation was developed, by which the biomass reached 37.02 g L−1at 28 day in the 5 L fermenter. The effects of inoculum with heterotrophic seeds into photoautotrophic cultivation were verified. By this strategy, the final content and productivity of hydrocarbon was 35.9% and 128.3 mg L−1d−1respectively in 1 L bubble column photo-bioreactors indoors, the highest ever reported. Furthermore, the heterotrophic seeds were successfully applied to photoautotrophic cultivation in 3 L flat panel photo-bioreactors and 120 L open circle ponds outdoors, in which the final content and productivity of hydrocarbon were 27.85% and 35.06 mg L−1d−1, indicating an efficient and feasible approach for producing biofuel fromB. braunii.