Biocompatibility of polyethersulfone membranes for cell culture systems
Biocompatibility of polyethersulfone membranes for cell culture systems
复制标题
DOI：
10.1002/elsc.201000125
复制
发表时间：
2011-12-01
影响因子：
2.7
通讯作者：
Sahebjamee, Foroud
中科院分区：
文献类型：
--
作者：
Azadbakht, Mehri;Madaeni, Sayed S.;Sahebjamee, Foroud
聚醚砜（PES）膜具有高的化学稳定性、热稳定性和机械稳定性，可用于低成本的膜制备。在这项研究中，PES膜和细胞培养插入物的制备和优化用于细胞培养系统。寻找具有高性能的新型膜是促进进一步组织培养应用的重要一步。比较了不透明膜、透明PES膜和商品膜的性能。倒置光学显微镜观察细胞及细胞膜结构。PES膜的细胞相容性通过培养HepG2细胞（一种人肝（癌）细胞系）来评价。评价细胞活力和粘附。通过测量吲哚菁绿色（ICG）的摄取和通过过碘酸希夫（PAS）染色来确定细胞的生物活性。虽然不透明和透明PES膜的形态与商业膜的形态不同，但它们表现出相似的性能。细胞呈圆形或椭圆形，并粘附在膜上形成三维聚集体。检测细胞对ICG的摄取，PAS染色鉴定糖原储备。在PES膜上培养的细胞的活性证实了所制备的膜的可接受的性质。自制的细胞培养插入物与商品化的细胞培养插入物相比，表现出令人满意的性能。
Polyethersulfone (PES) membranes with high chemical, thermal, and mechanical stability can be used for low-cost membrane preparation. In this study, PES membranes and cell culture inserts were prepared and optimized for utilization in cell culture systems. Finding new membranes with high performance is a vital step to promote further tissue culture applications. The performances of non-transparent, transparent PES, and commercial membranes were compared. The structures of the cells and membranes were observed by inverted optical microscopy. The cytocompatibility of PES membranes was evaluated by culturing HepG2 cells, a human liver (carcinoma) cell line. Cell viability and adhesion were evaluated. The biological activity of the cells was determined by measuring the uptake of indocyanine green (ICG) and by periodic acid Schiff (PAS) staining. Although the morphology of the non-transparent and transparent PES membranes was different from that of the commercial membranes, they demonstrated similar performances. The cells showed a round or oval shape and adhered to form 3D aggregates on the membrane. Cellular uptake of ICG was detected, and glycogen storage was identified by PAS staining. The activity of the cells cultured on the PES membranes confirmed the acceptable properties of the prepared membranes. The homemade cell culture inserts showed satisfactory performance compared with the commercial ones.