CATALYTIC ACTIVITY OF NATIVE ENZYMES DURING CAPILLARY ELECTROPHORESIS - AN ENZYMATIC MICROREACTOR
CATALYTIC ACTIVITY OF NATIVE ENZYMES DURING CAPILLARY ELECTROPHORESIS - AN ENZYMATIC MICROREACTOR
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DOI：
10.1021/jo00072a039
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发表时间：
1993-09-24
影响因子：
3.6
通讯作者：
WHITESIDES, GM
中科院分区：
文献类型：
--
作者：
AVILA, LZ;WHITESIDES, GM
这项工作评估了酶塞的使用，在非变性条件下在电泳毛细管中迁移，将底物（可以作为单独的塞注射到毛细管上或包含在电泳缓冲液中）转化为产物。使用两个系统演示了这一概念：使用葡萄糖-6-磷酸脱氢酶（G6PDH，EC 1.1.1.49）和NAD或NADP作为辅因子，将葡萄糖-6-磷酸（glc-6-P）不可逆氧化为6-磷酸葡萄糖酸，以及使用酵母乙醇脱氢酶（YADH，EC 1.1.1.1）和NAD（H）作为辅因子，将乙醇可逆转化为乙醛。辅因子。这些程序说明了电泳毛细管作为微反应器的用途，其中反应物和产物根据电泳迁移率的差异相互接触和脱离。它提供了一种在微尺度上操纵酶和酶催化反应的有用方法。
This work evaluates the use of a plug of enzyme, migrating in an electrophoresis capillary under nondenaturing conditions, to convert substrate (which may be injected onto the capillary as a separate plug or included in the electrophoresis buffer) to product. This concept is demonstrated using two systems: the irreversible oxidation of glucose-6-phosphate (glc-6-P) to 6-phosphogluconate using glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) and either NAD or NADP as cofactor, and the reversible conversion of ethanol to acetaldehyde using yeast alcohol dehydrogenase (YADH, EC 1.1.1.1) and NAD(H) as cofactor. These procedures illustrate the use of the electrophoresis capillary as a microreactor in which reactants and products are moved into and out of contact with one another based on differences in electrophoretic mobilities. It offers a useful approach to the manipulation of enzymes and enzyme-catalyzed reactions on a microscale.