Expression of CPI-17 in smooth muscle during embryonic development and in neointimal lesion formation
Expression of CPI-17 in smooth muscle during embryonic development and in neointimal lesion formation
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DOI：
10.1007/s00418-009-0604-2
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发表时间：
2009-08-01
影响因子：
2.3
通讯作者：
Eto, Masumi
中科院分区：
文献类型：
--
作者：
Kim, Jee In;Young, Garbo D.;Eto, Masumi
肌球蛋白轻链磷酸酶（MLCP）的抑制蛋白CPI-17决定平滑肌（SM）收缩的Ca 2+敏感性。CPI-17在成熟SM细胞中高度表达，但表达水平在病理条件下变化。在这里，我们确定了CPI-17的表达在胚胎SM组织和动脉新生内膜病变使用免疫组织化学。如在成年动物中所见，在小鼠胚胎切片的SM组织中检测到CPI-17的主要表达，而MLCP普遍表达。与SM α-肌动蛋白相比，CPI-17在胚胎第10天至第14天动脉SM中的表达增加了一倍。CPI-17与SM α-actin等SM标记蛋白一样，在胚胎心脏中表达，在E17表达下调。在成年大鼠中，CPI-17的表达水平降低到30%，在损伤的大鼠主动脉的新生内膜相比，SM层，而MLCP的表达在这两个区域是不变的。与其他SM蛋白不同，CPI-17在小鼠胚胎的非SM器官中检测到，例如胚胎神经元和上皮。因此，CPI-17表达响应于SM细胞的表型转变而被可逆地控制，所述表型转变将信号限制到分化的SM细胞和特定细胞类型。
Ca2+ sensitivity of smooth muscle (SM) contraction is determined by CPI-17, an inhibitor protein for myosin light chain phosphatase (MLCP). CPI-17 is highly expressed in mature SM cells, but the expression level varies under pathological conditions. Here, we determined the expression of CPI-17 in embryonic SM tissues and arterial neointimal lesions using immunohistochemistry. As seen in adult animals, the predominant expression of CPI-17 was detected at SM tissues on mouse embryonic sections, whereas MLCP was ubiquitously expressed. Compared with SM alpha-actin, CPI-17 expression doubled in arterial SM from embryonic day E10 to E14. Like SM alpha-actin and other SM marker proteins, CPI-17 was expressed in embryonic heart, and the expression was down-regulated at E17. In adult rat, CPI-17 expression level was reduced to 30% in the neointima of injured rat aorta, compared with the SM layers, whereas the expression of MLCP was unchanged in both regions. Unlike other SM proteins, CPI-17 was detected at non-SM organs in the mouse embryo, such as embryonic neurons and epithelium. Thus, CPI-17 expression is reversibly controlled in response to the phenotype transition of SM cells that restricts the signal to differentiated SM cells and particular cell types.