Classical noncholinergic neurotransmitters and the vesicular transport system for acetylcholine.
Classical noncholinergic neurotransmitters and the vesicular transport system for acetylcholine.
复制标题
经典的非胆碱能神经递质和乙酰胆碱的囊泡运输系统。
DOI：
10.1111/j.1471-4159.1993.tb03534.x
复制
发表时间：
1993
影响因子：
4.7
通讯作者：
Parsons,SM
中科院分区：
文献类型：
--
作者：
Clarkson,ED;Bahr,BA;Parsons,SM
乙酰胆碱转运体对乙酰胆碱的亲和力和特异性如此之低，以至于它可能无法与其他神经递质进行选择。研究了经典非胆碱能神经递质与从电器官中提纯的胆碱能突触小泡之间的潜在相互作用。不存在主动转运[~3H]5-羟色胺、[~3H]去甲肾上腺素或[~3H]谷氨酸。5-羟色胺、去甲肾上腺素和N-乙酰天门冬氨酸谷氨酸可抑制小泡主动转运[~3H]乙酰胆碱。此前，多巴胺曾被证明会抑制运输。谷氨酸和γ-氨基丁酸不抑制[~3H]-乙酰胆碱的主动转运。去甲肾上腺素在这一作用上与[~3H]乙酰胆碱具有竞争性。5-羟色胺、去甲肾上腺素和多巴胺抑制[~3H]维沙米考与囊泡的结合，而多巴胺是乙酰胆碱转运体的这种变构配体结合的竞争性抑制物。结果表明，乙酰胆碱转运体不转运任何其他经典的神经递质，但5-羟色胺、去甲肾上腺素和多巴胺与乙酰胆碱结合。
The acetylcholine transporter exhibits such low affinity and specificity for acetylchoiine that it appeared possible it could fail to select against other neurotransmitters. Potential interactions of classical noncholinergic neurotransmitters with cholinergic synaptic vesicles purified from electric organ were studied. No active transport of [3H]serotonin, [3H]noradrenaline, or [3H]glutamate occurred. Serotonin, noradrenaline, andN‐acetylaspartyl glutamate inhibited active transport of [3H]acetylcholine by the vesicles. Dopamine previously had been shown to inhibit transport. Glutamate and γ‐aminobutyric acid were shown here not to inhibit active transport of [3H]‐acetylcholine. Noradrenaline was competitive with respect to [3H]acetylcholine in this effect. Serotonin, noradrenaline, and dopamine inhibited binding of [3H]vesamicol to the vesicles, and dopamine was a competitive inhibitor of the binding of this allosteric ligand of the acetylcholine transporter. The results indicate that the acetylcholine transporter does not transport any other classical neurotransmitter, but serotonin, noradrenaline, and dopamine bind to the acetylcholine site.