DNA-SEQUENCE OF THE GENE ENCODING THE E-ALPHA IA POLYPEPTIDE OF THE BALB/C MOUSE
DNA-SEQUENCE OF THE GENE ENCODING THE E-ALPHA IA POLYPEPTIDE OF THE BALB/C MOUSE
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DOI：
10.1126/science.6815800
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
HOOD, L
中科院分区：
文献类型：
--
作者：
MCNICHOLAS, J;STEINMETZ, M;HOOD, L
一个3.4千碱基的DNA片段已被测序，该片段包含编码来自BALB/c小鼠的Ia（I区相关）抗原的Eα链的基因。它包含至少三个外显子，与Eα链的主要结构域-两个外部结构域α1和α2以及跨膜-胞质结构域相关。小鼠Eα基因的编码序列在DNA和蛋白质水平上与人E α基因具有惊人的同源性。翻译的α2外显子与β2-微球蛋白、免疫球蛋白恒定区结构域和移植抗原的某些结构域具有显著的相似性。这些观察结果和其他人的观察结果表明，Ia抗原，移植抗原和免疫球蛋白基因家族共享一个共同的祖先。
A 3.4-kilobase DNA fragment containing the gene coding for the Eαchain of an Ia (I region-associated) antigen from the BALB/c mouse has been sequenced. It contains at least three exons, which correlate with the major structural domains of the Eαchain—the two external domains α1 and α2, and the transmembrane-cytoplasmic domain. The coding sequence of the mouse Eαgene shows striking homology to its human counterpart at the DNA and protein levels. The translated α2 exon demonstrates significant similarity to β2-microglobulin, to immunoglobulin constant region domains, and to certain domains of transplantation antigens. These observations and those of others suggest that the Ia antigen, transplantation antigen, and immunoglobulin gene families share a common ancestor.