Application of microarray-based method for methylenetetrahydrofolate reductase (MTHFR) polymorphisms in the risk of gastric carcinoma in east China population.
Application of microarray-based method for methylenetetrahydrofolate reductase (MTHFR) polymorphisms in the risk of gastric carcinoma in east China population.
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DOI：
10.1166/jnn.2007.693
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发表时间：
2007-09
影响因子：
--
通讯作者：
Song Li;Meiju Ji;N. He;Zuhong Lu
中科院分区：
文献类型：
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作者：
Song Li;Meiju Ji;N. He;Zuhong Lu
采用微阵列技术探讨中国人群中亚甲基四氢叶酸还原酶（MTHFR）基因多态性（C677T和A1298C）与胃癌发病风险的关系。从170例胃癌患者和140例年龄和性别匹配的对照组中分离出基因组DNA。C677T基因型的频率分别为：对照组CC（47.9%）、CT（40%）、CT(12.1%)，胃癌组CC（35.9%）、CT（45.9%）、TT（18.2%）。与携带677CC基因型的个体相比，携带677CT + TT或677TT基因型的个体发生胃癌的风险增加了1.67倍（95% CI: 1.06-2.64）或2.67倍（95% CI: 1.382-5.341）。两组间MTHFR基因A1298C等位基因的基因型差异无统计学意义。我们发现MTHFR C677T和A1298C多态性在胃癌发生风险上存在联合作用。我们的研究结果表明，MTHFR基因的单核苷酸多态性与中国东部人群胃癌的发生风险有关。
The microarrays were fabricated to explore the relationship between methylenetetrahydrofolate reductase (MTHFR) polymorphisms (C677T and A1298C) and risk of gastric carcinoma in Chinese population. The genomic DNA was isolated from 170 patients with gastric carcinoma and 140 age- and sex-matched control subjects. The frequencies of C677T genotype were: CC (47.9%), CT (40%), CT (12.1%) in control group and CC (35.9%), CT (45.9%), TT (18.2%) in gastric carcinoma group, respectively. The individuals with 677CT + TT or 677TT genotypes had a 1.67-fold (95% CI: 1.06-2.64) or 2.67-fold (95% CI: 1.382-5.341) increased risk of developing gastric carcinoma compared with those carrying 677CC genotype. The genotype of MTHFR gene A1298C allele was not significantly different between the two groups. We found that a joint effect exits between the MTHFR C677T and A1298C polymorphism on the risk of gastric carcinoma. Our results show that the single nucleotide polymorphisms in the MTHFR gene are associated with the risk of gastric carcinoma in the east China population.