Nuclear matrix-bound deoxyribonucleic acid synthesis: an in vitro system.
Nuclear matrix-bound deoxyribonucleic acid synthesis: an in vitro system.
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DOI：
10.1021/bi00269a021
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发表时间：
1982-12
期刊：
影响因子：
2.9
通讯作者：
H. C. Smith;R. Berezney
中科院分区：
文献类型：
--
作者：
H. C. Smith;R. Berezney
已从再生大鼠肝脏中分离出一种核基质结合的体外复制系统，该系统利用 α 聚合酶和基质附着的 DNA，并针对体外要求进行了表征。 β-聚合酶是体外基质系统的次要成分。有证据表明 α 聚合酶与核基质的天然关联，以及在部分肝切除术后的增殖反应期间平行诱导基质结合的 α 聚合酶和体内 DNA 合成。我们的数据表明，α-聚合酶与基质的附着可能是功能性复制体组装的重要步骤。我们还证明了复制前和复制后期核和基质结合β-聚合酶活性的增加，并在可能的基因组筛选方面讨论了这些结果。
A nuclear matrix-bound in vitro replicational system that utilizes alpha-polymerase and matrix-attached DNA has been isolated from regenerating rat liver and characterized for in vitro requirements. beta-Polymerase is a minor component of the matrix in vitro system. Evidence is presented for the native association of alpha-polymerase with the nuclear matrix and for the parallel induction of matrix-bound alpha-polymerase and in vivo DNA synthesis during the proliferative response following partial hepatectomy. Our data suggest that the attachment of alpha-polymerase to the matrix may be an important step in the assembly of functional replisomes. We also demonstrate prereplicative and late-replicative rises in nuclear and matrix-bound beta-polymerase activity and discuss these results in terms of possible genome screening.