Secretagogue response of goblet cells and columnar cells in human colonic crypts
Secretagogue response of goblet cells and columnar cells in human colonic crypts
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DOI：
10.1152/ajpcell.1999.277.3.c501
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发表时间：
1999-09-01
影响因子：
5.5
通讯作者：
Halm, ST
中科院分区：
文献类型：
--
作者：
Halm, DR;Halm, ST
人结肠隐窝杯状细胞和柱状细胞的促分泌反应。Am. J.Physiol.277（Cell Physiol.46）：C501-C522，1999。Lieberkuhn隐窝分离自人结肠，差示干涉显微镜区分杯状和柱状细胞。用卡巴胆碱（CCh，100 μ M）或组胺（10 μ M）激活，从杯状颗粒中释放内容物。用前列腺素E-2（PGE（2），5 μ M）或腺苷（10 μ M）刺激不释放杯状颗粒，但引起柱状细胞的顶缘后退。在用CCh或组胺刺激期间，杯状体积损失（类似于160 fl/细胞），但用PGE（2）或腺苷则没有。四分之三的杯状细胞对CCh有反应，但仅释放30%的杯状体积。杯状体积释放的半衰期为3.7分钟。PGE 2刺激延长的液体分泌，达到接近350 pl/min的速率。在最大PGE 2刺激期间，柱状细胞损失了接近50%的顶端体积，半衰期为3.3分钟。在溃疡性结肠炎患者的隐窝中，杯状细胞对CCh释放杯状体积高度敏感。这些结果支持分别调节从杯状细胞和柱状细胞的粘液分泌，控制机制限制总释放的粘液储存。
Secretagogue response of goblet cells and columnar cells in human colonic crypts. Am. J. Physiol. 277 (Cell Physiol. 46): C501-C522, 1999.-Crypts of Lieberkuhn were isolated from human colon, and differential interference contrast microscopy distinguished goblet and columnar cells. Activation with carbachol (CCh, 100 mu M) or histamine (10 mu M) released contents from goblet granules. Stimulation with prostaglandin E-2 (PGE(2), 5 mu M) or adenosine (10 mu M) did not release goblet granules but caused the apical margin of columnar cells to recede. Goblet volume was lost during stimulation with CCh or histamine (similar to 160 fl/cell), but not with PGE(2) or adenosine. Three-quarters of goblet cells were responsive to CCh but released only 30% of goblet volume. Half-time for goblet volume release was 3.7 min. PGE2 stimulated a prolonged fluid secretion that attained a rate of similar to 350 pl/min. Columnar cells lost similar to 50% of apical volume during maximal PGE2 stimulation, with a half-time of 3.3 min. In crypts from individuals with ulcerative colitis, goblet cells were hypersensitive to CCh for release of goblet volume. These results support separate regulation for mucus secretions from goblet cells and from columnar cells, with control mechanisms restricting total release of mucus stores.