Identification of a TH2-high psoriasis cluster based on skin biomarker analysis in a Chinese psoriasis population
Identification of a TH2-high psoriasis cluster based on skin biomarker analysis in a Chinese psoriasis population
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基于中国银屑病人群皮肤生物标志物分析识别 T(H)2 高银屑病簇
DOI：
10.1111/jdv.16563
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发表时间：
2020-07-27
影响因子：
9.2
通讯作者：
Yao, Z.
中科院分区：
文献类型：
--
作者：
Chen, J.;Li, C.;Yao, Z.
背景：银屑病是一种免疫介导的慢性炎症性疾病，具有多种表型。然而，其生物学多样性在中国银屑病人群中尚未得到充分表征。
目的：利用中国银屑病人群皮损皮肤活检标本的基因表达谱来表征银屑病的生物学异质性。
方法：通过实时定量聚合酶链反应阵列、组织学评估和流式细胞术对中国银屑病患者（n = 40）、特应性皮炎（AD）患者（n = 25）以及年龄匹配的健康对照者（n = 19）的皮损组织和血液样本进行研究。利用银屑病患者的基因表达谱进行无监督层次聚类分析。
结果：确定了两个不同的银屑病聚类。两个聚类都显示出高的辅助性T细胞17（TH17）活化。一个聚类（连续40例银屑病患者中的6例）显示皮损中有强烈的辅助性T细胞2（TH2）成分，发病早且外周血嗜酸性粒细胞水平低。白细胞介素 - 4（IL - 4）、白细胞介素 - 13（IL - 13）、白细胞介素 - 25（IL - 25）、白细胞介素 - 31（IL - 31）和胸腺基质淋巴细胞生成素（TSLP）基因诱导显著更高是这组银屑病患者的典型特征，甚至与特应性皮炎患者相比也是如此。两个银屑病聚类都以中性粒细胞微脓肿形成为特征。在组织学上，TH2高表达的银屑病聚类显示血管周围嗜酸性粒细胞百分比低。
结论：确定了两个不同的银屑病聚类。其中一个发病早且嗜酸性粒细胞水平低，表明存在TH17极化和强烈的TH2成分。这些结果为进一步阐明中国人群银屑病的发病机制奠定了基础。
Background: Psoriasis is an immune-mediated, chronic inflammatory disease with diverse phenotypes. However, its biological diversity has not been well-characterized in Chinese psoriasis population.Objectives: To characterize psoriasis biological heterogenicity using gene expression profiles of lesional skin biopsy specimens in a Chinese psoriasis population.Methods: Lesional tissues and blood samples from Chinese psoriasis patients (n = 40), atopic dermatitis (AD) patients (n = 25) and age-matched healthy controls (n = 19) were investigated by using real-time PCR array, histological evaluation and flow cytometry. Unsupervised hierarchical clustering was performed using gene expression profiles of patients with psoriasis.Results: Two distinct psoriasis clusters were identified. Both clusters indicated high T(H)17 activation. One cluster (n = 6 of 40 consecutive psoriasis patients) indicated a strong T(H)2 component in skin lesions, with early onset and low peripheral blood eosinophil level. Significantly higher IL-4, IL-13, IL-25, IL-31 and TSLP gene induction typified this cluster of psoriasis patients, even compared with AD patients. Both psoriasis clusters were characterized by neutrophilic microabscess formation. Histologically, the T(H)2 high psoriasis cluster indicated a low percentage of perivascular eosinophils.Conclusions: Two distinct psoriasis clusters were identified. One presented early onset and a low eosinophil level, indicating T(H)17 polarization and a strong T(H)2 component. These results laid the foundation for further demonstrating the pathogenesis of psoriasis in Chinese population.