An immunological electrospun scaffold for tumor cell killing and healthy tissue regeneration
An immunological electrospun scaffold for tumor cell killing and healthy tissue regeneration
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用于杀死肿瘤细胞和健康组织再生的免疫电纺支架
DOI：
10.1039/c8mh00704g
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发表时间：
2018-11-01
影响因子：
13.3
通讯作者：
Shi, Qin
中科院分区：
文献类型：
--
作者：
Liu, Xingzhi;Zhang, Hongbo;Shi, Qin
近年来，基于抗体的肿瘤免疫治疗引起了广泛的研究兴趣，但抗体的易分布性和爆发性释放极大地限制了其应用。此外，组织清除后，健康组织再生是癌症治疗的另一个挑战。本研究将小鼠抗人CD 40单克隆抗体（CD 40 mAb）通过多巴胺（PDA）基序（PDA-PDA-CD 40 mAb）掺入聚乳酸（PLLA）电纺纤维中，构建了一种特异性免疫组织工程支架。免疫荧光染色证明，CD 40 mAb成功掺入到电纺纤维支架表面，PLLA-PDA-CD 40 mAb支架通过局部释放CD 40 mAb而具有抗肿瘤作用。因此，这种免疫电纺支架具有很好的潜力，可以发展成为一种强有力的工具，用于局部肿瘤治疗，这是第一次在这方面的报告。
Antibody-based cancer immune therapy has attracted lots of research interest in recent years; however, it is greatly limited by the easy distribution and burst release of antibodies. In addition, after the clearance of the tissue, healthy tissue regeneration is another challenge for cancer treatment. Herein, we have developed a specific immunological tissue engineering scaffold using the agonistic mouse anti-human CD40 antibody (CD40mAb) incorporated into poly(l-lactide) (PLLA) electrospun fibers through the dopamine (PDA) motif (PLLA-PDA-CD40mAb). CD40mAb is successfully incorporated onto the surface of the electrospun fibrous scaffold, which is proved by immunofluorescence staining, and the PLLA-PDA-CD40mAb scaffold has an anti-tumor effect by locally releasing CD40mAb. Therefore, this immunological electrospun scaffold has very good potential to be developed as a powerful tool for localized tumor treatment, and this is the first to be reported in this area.