Optimization of Extraction Parameters for Enhanced Production of Ovotransferrin from Egg White for Antimicrobial Applications
Optimization of Extraction Parameters for Enhanced Production of Ovotransferrin from Egg White for Antimicrobial Applications
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DOI：
10.1155/2015/934512
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发表时间：
2015-01-01
影响因子：
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通讯作者：
Haque, Shafiul
中科院分区：
文献类型：
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作者：
Alshammari, Eyad M. A.;Khan, Saif;Haque, Shafiul
卵转铁蛋白是蛋清中含量第二高的蛋白质（约占鸡蛋总蛋白的12-13%），仅次于卵清蛋白。卵转铁蛋白是一种有效的天然抗菌剂，因为它具有抗菌、抗真菌和抗病毒的特性，也是鸡蛋中发现的主要金属结合蛋白，这使其成为工业上重要的蛋白质。利用蛋白的金属（铁）结合特性，从蛋白中提取卵黄转铁蛋白。本研究采用两种不同来源的鸡蛋（本地家庭新鲜鸡蛋和商店家禽鸡蛋）进行比较，采用响应面法进行实验设计和数据分析。以蛋清中卵黄转铁蛋白得率最高为目标，优化了提取条件：乙醇浓度为% (v/v)， pH和体积为25 mM FeCl3/50mL的蛋清。在最佳提取条件下，最大得率为85 +/- 2.5%。产率根据卵黄转铁蛋白在100 mL 1.5倍稀释的蛋清溶液中的理论值（934 mg）计算。我们的研究结果表明，高效的下游加工可以降低这种有前途的酶的整个生产过程的成本，使其成为现有化学合成抗菌剂的天然和经济的替代品。
Ovotransferrin is the second most abundant protein (similar to 12-13% of the total egg protein) in egg white after ovalbumin. Ovotransferrin is a potent natural antimicrobial agent as it possesses antibacterial, antifungal, and antiviral properties and is also the major metal binding protein found in egg, which makes it an industrially important protein. Ovotransferrin was extracted from egg white using its metal (iron) binding properties. In the present study, eggs from two different sources were used (fresh local eggs from domestic household source and poultry eggs from shops) to compare the results and Response Surface Methodology was used for the experiment design and data analysis. The following extraction conditions were optimized so as to maximize the yield of ovotransferrin from egg white: ethanol % (v/v) and pH and volume (mL) of 25 mM FeCl3/50mL of egg white. A maximum yield of similar to 85 +/- 2.5% was obtained near the optimum extraction conditions. The yield was calculated based on the theoretical value (934 mg) of ovotransferrin in 100 mL of 1.5x diluted egg white solution. Our results suggest that efficient downstream processing may reduce the cost of overall production process of this promising enzyme, making it a natural and cost-effective alternative to the existing chemically synthesized antimicrobial agents.