Complete nucleotide sequence of the streptococcal C5a peptidase gene of Streptococcus pyogenes.
Complete nucleotide sequence of the streptococcal C5a peptidase gene of Streptococcus pyogenes.
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DOI：
10.1016/s0021-9258(19)39748-0
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发表时间：
1990-02
期刊：
影响因子：
--
通讯作者：
Chunsheng Chen;P. Cleary
中科院分区：
文献类型：
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作者：
Chunsheng Chen;P. Cleary
链球菌C5a肽酶（SCP）是最近发现的化脓性链球菌的毒力因子，其特异性地切割人血清趋化因子C5a的羧基末端附近，破坏其作为化学引诱物的能力。我们先前将SCP基因scpA定位于重组质粒pTT1的5.8 kb插入片段。在这里，我们提出了完整的核苷酸序列的scpA及其侧翼区。该基因起始于TTG密码子，由3501个碱基对组成，指定128，252道尔顿的前体蛋白。在scpA的上游发现类似于革兰氏阳性生物的启动子和核糖体结合位点的序列。预测的氨基酸序列揭示了31个残基的信号肽，推定的细胞壁跨越和膜锚域的存在。SCP的区域显示出与原核丝氨酸蛋白酶枯草杆菌蛋白酶的活性位点的形成所涉及的序列的显著相似性。Southern杂交研究结果表明，与scpA高度相似的序列存在于所有血清型的S中。化脓菌检测。
Streptococcal C5a peptidase (SCP), a recently discovered virulence factor of Streptococcus pyogenes, specifically cleaves the human serum chemotaxin C5a near its carboxyl terminus, destroying its ability to serve as a chemoattractant. We previously localized the SCP gene, scpA, to the 5.8-kb insert of the recombinant plasmid pTT1. Here we present the complete nucleotide sequence of scpA and its flanking regions. The gene initiates at a TTG codon and consists of 3501 base pairs, specifying a precursor protein of 128,252 daltons. Sequences resembling the promoter and ribosome-binding site of Gram-positive organisms are found upstream of scpA. The predicted amino acid sequence reveals the presence of a 31-residue signal peptide, putative cell wall spanning and membrane anchor domains. Regions of SCP show significant similarity to the sequences involved in the formation of the active site of the prokaryotic serine protease subtilisin. Results of Southern hybridization studies indicate that sequences highly similar to that of scpA are present in all serotypes of S. pyogenes tested.