Involvement of SRD2-mediated activation of snRNA transcription in the control of cell proliferation competence in Arabidopsis.
Involvement of SRD2-mediated activation of snRNA transcription in the control of cell proliferation competence in Arabidopsis.
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DOI：
10.1111/j.1365-313x.2005.02469.x
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发表时间：
2005-07
期刊：
影响因子：
--
通讯作者：
M. Ohtani;M. Sugiyama
中科院分区：
文献类型：
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作者：
M. Ohtani;M. Sugiyama
小核RNA（snRNA）基因的转录机制已在人类无细胞系统中得到广泛研究，但其在体内的生理功能尚未得到解决。本文证明了生理作用的激活剂的snRNA转录使用温度敏感的突变体拟南芥，srd2。srd2突变体的表型特征表明，SRD2基因参与控制的能力，在细胞增殖。SRD2基因编码与人SNAP50蛋白共享序列相似性的核蛋白，SNAP50蛋白是SNAPc的亚基并且是体外snRNA转录所需的。我们的结果，从srd2突变体中的snRNA表达的分析中获得的，表明SRD2蛋白的功能在上调snRNA基因的转录，其启动子包含上游序列元件，以提高细胞增殖能力。
The transcription machinery of small nuclear RNA (snRNA) genes has been investigated extensively in human cell-free systems, but its physiological function in vivo has not been addressed. This paper demonstrates the physiological role of an activator of snRNA transcription using a temperature-sensitive mutant of Arabidopsis thaliana, srd2. Phenotypic characteristics of the srd2 mutant suggest that the SRD2 gene participates in the control of competence in cell proliferation. The SRD2 gene encodes a nuclear protein that shares sequence similarity with the human SNAP50 protein, which is a subunit of SNAPc and is required for snRNA transcription in vitro. Our results, obtained from analysis of snRNA expression in the srd2 mutant, indicate that the SRD2 protein functions in the upregulation of transcription of snRNA genes, the promoters of which contain the upstream sequence element, to elevate cell proliferation competence.