A minimum of four human class II alpha-chain genes are encoded in the HLA region of chromosome 6.
A minimum of four human class II alpha-chain genes are encoded in the HLA region of chromosome 6.
复制标题
6 号染色体的 HLA 区域至少编码 4 个人类 II 类 α 链基因。
DOI：
10.1038/304174a0
复制
发表时间：
1983
期刊：
影响因子：
64.8
通讯作者：
Strominger,JL
中科院分区：
文献类型：
--
作者：
Auffray,C;Kuo,J;DeMars,R;Strominger,JL
人类的主要组织相容性复合体（MHC），也称为HLA区域，位于6号染色体的短臂上，编码参与免疫过程的抗原1。II类HLA抗原由两条非共价结合的多肽链组成，一条分子量为34，000（α），另一条分子量为29，000（β）2。β链的广泛多态性允许将相应的β基因遗传定位到HLA-DR区域3 -5。HLA-DRα链的cDNA克隆已被用于使用小鼠-人类体细胞杂交将非多态性DRα链基因定位到染色体6上6。同样，DRα链基因7通过原位杂交实验定位于6号染色体着丝粒的HLA-A、-B和-C位点的短臂上8。我们分离了一个与DRα链相关的cDNA克隆，编码II类抗原DCα链9。我们在这里描述了如何使用DCα克隆通过交叉杂交找到两个或三个额外的α链基因，以及如何使用人类淋巴母细胞系（LCL）的HLA抗原缺失突变体来确定这些额外的II类抗原α链基因也位于HLA区域。
The major histocompatibility complex (MHC) in man, also called the HLA region, is located on the short arm of chromosome 6 and encodes antigens involved in immunological processes1. The class II HLA antigens consist of two noncovalently associated polypeptide chains, one of molecular weight 34,000 (α) and the other of molecular weight 29,000 (β)2. The extensive polymorphism of theβchain(s) has allowed the genetic mapping of the correspondingβgene(s) to the HLA-DR region3–5. cDNA clones for the HLA-DRαchain have been used to map the non-polymorphic DRα-chain gene to chromosome 6 using mouse–human somatic cell hybrids6. Similarly, the DRα-chain gene7has been mapped to the short arm of chromosome 6 centromeric to the HLA-A, -B and -C loci byin situhybridization experiments8. We isolated a cDNA clone that is related to the DRαchain and encodes the class II antigen DCαchain9. We describe here how this DCαclone was used to find two or three additionalα-chain genes by cross-hybridization and how HLA-antigen loss mutants of a human lymphoblastoid cell line (LCL) were used to ascertain that these additional class II antigenα-chain genes are also located in the HLA region.