Effect of partial outlet obstruction of the rabbit urinary bladder on ryanodine binding to microsomal membranes.
Effect of partial outlet obstruction of the rabbit urinary bladder on ryanodine binding to microsomal membranes.
复制标题
兔膀胱部分出口梗阻对兰尼定与微粒体膜结合的影响。
DOI：
10.1016/0306-3623(94)90191-0
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Wein,AJ
中科院分区：
文献类型：
--
作者：
Levin,RM;Levin,SS;Zderic,SA;Saito,M;Yoon,JY;Wein,AJ
1.兔膀胱出口部分梗阻可显著增强ryanodine抑制电场刺激收缩的能力。目前的研究确定了出口阻塞对ryanodine与微粒体膜结合的影响。2.结果表明：（i）Ryanodine结合是线性的，显示一组Ryanodine结合位点（Bmax= 28+/-3.5 fmol/mg蛋白; Kd= 4.2 nM）。(ii)梗阻后1天和3天，总兰尼碱结合率略有增加。(iii)在阻塞后第5天和第7天，结合增加约4倍。(iv)在任何时间段均未观察到Kd变化。3.这些研究的结果表明，继发于部分出口梗阻的平滑肌肥大诱导细胞内钙在介导对场刺激的收缩反应中的作用显著增加。
1. Partial outlet of obstruction of the rabbit urinary bladder results in a significant increase in the ability of ryanodine to inhibit field stimulated contraction. The current study determined the effect of outlet obstruction on ryanodine binding to microsomal membranes. 2. The results demonstrate:(i) Ryanodine binding is linear showing one set of ryanodine binding sites (Bmax= 28+/-3.5 fmol/mg protein; Kd= 4.2 nM).(ii) Total ryanodine binding was slightly increased at 1 and 3 day following obstruction.(iii) Binding increased approx 4-fold at 5 and 7 days post obstruction.(iv) No change in the Kds were noted at any time period. 3. The results of these studies indicate that smooth muscle hypertrophy secondary to partial outlet obstruction induces a marked increase in the role of intracellular calcium in the mediation of the contractile response to field stimulation.