Steerable-filter based quantification of axonal populations at the developing optic chiasm reveal significant defects in Slit2(-/-) as well as Slit1(-/-)Slit2(-/-) embryos.
Steerable-filter based quantification of axonal populations at the developing optic chiasm reveal significant defects in Slit2(-/-) as well as Slit1(-/-)Slit2(-/-) embryos.
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DOI：
10.1186/1471-2202-14-9
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发表时间：
2013-01-15
期刊：
影响因子：
2.4
通讯作者：
Price DJ
中科院分区：
文献类型：
--
作者：
Down M;Willshaw DA;Pratt T;Price DJ
以前的研究表明，轴突引导蛋白Slit1和Slit2共同作用，在腹侧间脑中线的正确位置建立视交叉。这是基于这样的观察结果：尽管Slit1和Slit2都在腹中线附近表达，但仅有Slit1和Slit2基因缺陷的小鼠在视交叉上表现出很少或没有轴突引导缺陷，而同时缺乏Slit1和Slit2的胚胎在视交叉的正常位置之前发育出大量额外的交叉。在这里，我们使用可定向过滤器来量化野生型、SLIT1−/−、SLIT2−/−和SLIT1−/−SLIT2−/−胚胎交叉处轴突种群的关键属性。我们成功地将定向滤波算法应用于单眼标记的胚胎视网膜轴突图像，这些图像在穿过中线后不久就被标记。我们结合了来自相同基因的多个胚胎的数据，并对不同基因组间的轴突分布、方向和曲率进行了统计比较。我们将轴突分析的数据与Slit1和Slit2的表达数据进行了比较。结果显示，SLIT2−/−和SLIT1−/−SLIT2−/−突变体的交叉处许多轴突的位置发生了错位和相应的前移。Slit1−/−突变体的交叉部位几乎没有轴突缺陷。我们发现SLIT1、−/−、SLIT2、−/−和SLIT1−/−突变体的交叉缺陷与以前报道的类似。此外，我们发现了以前没有报道的缺陷，这些缺陷是由于Slit2本身的丢失造成的。这表明了定量方法对复杂通路分析的价值，并表明Slit2可以单独作用于控制穿过腹侧间脑中线的视网膜轴突的各个方面。
Previous studies have suggested that the axon guidance proteins Slit1 and Slit2 co-operate to establish the optic chiasm in its correct position at the ventral diencephalic midline. This is based on the observation that, although both Slit1 and Slit2 are expressed around the ventral midline, mice defective in either gene alone exhibit few or no axon guidance defects at the optic chiasm whereas embryos lacking both Slit1 and Slit2 develop a large additional chiasm anterior to the chiasm’s normal position. Here we used steerable-filters to quantify key properties of the population of axons at the chiasm in wild-type, Slit1−/−, Slit2−/− and Slit1−/−Slit2−/− embryos. We applied the steerable-filter algorithm successfully to images of embryonic retinal axons labelled from a single eye shortly after they have crossed the midline. We combined data from multiple embryos of the same genotype and made statistical comparisons of axonal distributions, orientations and curvatures between genotype groups. We compared data from the analysis of axons with data on the expression of Slit1 and Slit2. The results showed a misorientation and a corresponding anterior shift in the position of many axons at the chiasm of both Slit2−/− and Slit1−/−Slit2−/− mutants. There were very few axon defects at the chiasm of Slit1−/− mutants. We found defects of the chiasms of Slit1−/−Slit2−/− and Slit1−/− mutants similar to those reported previously. In addition, we discovered previously unreported defects resulting from loss of Slit2 alone. This indicates the value of a quantitative approach to complex pathway analysis and shows that Slit2 can act alone to control aspects of retinal axon routing across the ventral diencephalic midline.
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影响因子：
3.5
作者：
Plachez, Celine;Andrews, William;Sundaresan, Vasi
通讯作者：
Sundaresan, Vasi
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
16.2
作者：
Plump, AS;Erskine, L;Tessier-Lavigne, M
通讯作者：
Tessier-Lavigne, M
影响因子：
2.7
作者：
Thompson, Hannah;Barker, David;Erskine, Lynda
通讯作者：
Erskine, Lynda
影响因子：
3.7
作者：
Meijering, E;Jacob, M;Unser, M
通讯作者：
Unser, M