The contribution of the stomach to ethanol oxidation in the rat.
The contribution of the stomach to ethanol oxidation in the rat.
复制标题
胃对大鼠乙醇氧化的贡献。
DOI：
10.1016/0024-3205(87)90691-6
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发表时间：
1987
期刊：
影响因子：
6.1
通讯作者：
Lieber,CS
中科院分区：
文献类型：
--
作者：
Caballeria,J;Baraona,E;Lieber,CS
为了估计可以在胃中氧化的乙醇的量，通过给予负荷剂量的乙醇（2g/kg体重I. V.）随后通过静脉内或胃内连续输注。根据维持约30 mM的稳定血液水平至少3小时所需的输注速率计算乙醇氧化速率。胃肠道乙醇浓度和总含量也保持稳定。灌胃时乙醇氧化速率比静脉输注时快19.3%（P<0.01）。当在主要管腔乙醇浓度（145 mM）下测量并按体重表示时，胃ADH活性占30 mM乙醇下肝脏活性的14%，表明胃ADH活性可以解释胃内给予乙醇时氧化速率增加的大部分原因。因此，在大鼠中由高Km ADH引起的胃乙醇氧化代表乙醇氧化总速率的显著部分，因此它是决定口服给药乙醇的生物利用度的因素之一。
To estimate the amount of ethanol that can be oxidized in the stomach, steady-state conditions were created in a group of fed rats by giving a loading dose of ethanol (2 g/kg body wt I.V.) followed by continuous infusion either intravenously or intragastrically. The rate of ethanol oxidation was calculated from the rate of infusion required to maintain steady blood levels of approximately 30 mM for at least 3 hours. Gastrointestinal ethanol concentrations and total contents also remained steady. The rate of ethanol oxidation was 19.3% faster during intragastric than during intravenous infusion (p<0.01). When measured at the prevailing luminal ethanol concentration (145 mM) and expressed per body weight, the gastric ADH activity represented 14% of the hepatic activity at 30 mM ethanol, sugesting that gastric ADH activity could account for most of the increased rate of oxidation when ethanol is given intragastrically. Thus, gastric ethanol oxidation by a high Km ADH in the rat represents a significant fraction of the total rate of ethanol oxidation and it is therefore one of the factors which determines the bioavailability of orally administered ethanol.