Detecting cell-secreted growth factors in microfluidic devices using bead-based biosensors
Detecting cell-secreted growth factors in microfluidic devices using bead-based biosensors
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DOI：
10.1038/micronano.2017.25
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发表时间：
2017-07-03
影响因子：
7.9
通讯作者：
Revzin, Alexander
中科院分区：
文献类型：
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作者：
Son, Kyung Jin;Gheibi, Pantea;Revzin, Alexander
微流控系统提供了一个有趣的替代标准的宏观规模的细胞培养，由于细胞和试剂的数量减少，以及改善的生理细胞局限于小体积。然而，可用于微流体装置内的细胞分泌分子的工具仍然有限。在本文中，我们描述了一个集成的微系统组成的微流控装置和荧光微珠为基础的检测肝细胞生长因子（HGF）和转化生长因子（TGF）-β 1的原代肝细胞分泌的测定。该微流体系统被设计成使用可渗透的水凝胶屏障将细胞培养室与感测室分开。细胞分泌的HGF和TGF-β 1通过水凝胶屏障扩散到相邻的传感通道中，并使用基于荧光微珠的传感器进行检测。传感微珠的特异性由抗体的选择来定义;因此，我们的微流体培养系统和传感微珠可以应用于各种细胞和细胞分泌因子。
Microfluidic systems provide an interesting alternative to standard macroscale cell cultures due to the decrease in the number of cells and reagents as well as the improved physiology of cells confined to small volumes. However, the tools available for cell-secreted molecules inside microfluidic devices remain limited. In this paper, we describe an integrated microsystem composed of a microfluidic device and a fluorescent microbead-based assay for the detection of the hepatocyte growth factor (HGF) and the transforming growth factor (TGF)-beta 1 secreted by primary hepatocytes. This microfluidic system is designed to separate a cell culture chamber from sensing chambers using a permeable hydrogel barrier. Cell-secreted HGF and TGF-beta 1 diffuse through the hydrogel barrier into adjacent sensing channels and are detected using fluorescent microbead-based sensors. The specificity of sensing microbeads is defined by the choice of antibodies; therefore, our microfluidic culture system and sensing microbeads may be applied to a variety of cells and cell-secreted factors.