Cyclic aristeromycin diphosphate ribose: A potent and poorly hydrolysable Ca2+-mobilising mimic of cyclic adenosine diphosphate ribose
Cyclic aristeromycin diphosphate ribose: A potent and poorly hydrolysable Ca2+-mobilising mimic of cyclic adenosine diphosphate ribose
复制标题
DOI：
10.1016/0014-5793(95)01515-9
复制
发表时间：
1996-02-05
期刊：
影响因子：
3.5
通讯作者：
Potter, BVL
中科院分区：
文献类型：
--
作者：
Bailey, VC;Fortt, SM;Potter, BVL
环状二磷酸阿里斯特霉素核糖是环状二磷酸腺苷核糖的碳环类似物，采用化学酶途径合成，涉及通过二苯基磷酰氯激活阿里斯特霉素 5'-磷酸。在海胆蛋匀浆中研究了这种新型类似物的钙释放特性。虽然环阿里斯霉素二磷酸核糖的钙释放曲线与环腺苷二磷酸核糖相似（EC(50) 值分别为 80 nM 和 30 nM），但它的降解速度明显更慢（t(1/2) 值分别为 170 分钟和 15 分钟），因此，环酸可能是研究环腺苷二磷酸核糖活性的有用工具。
Cyclic aristeromycin diphosphate ribose, a carbocyclic analogue of cyclic adenosine diphosphate ribose, was synthesised using a chemo-enzymatic route involving activation of aristeromycin 5'-phosphate by diphenyl phosphochloridate. The calcium-releasing properties of this novel analogue were investigated in sea urchin egg homogenates. While cyclic aristeromycin diphosphate ribose has a calcium release profile similar to that of cyclic adenosine diphosphate ribose (EC(50) values are 80 nM and 30 nM, respectively), it is degraded significantly more slowly (t(1/2) values are 170 min and 15 min, respectively) acid may, therefore, be a useful tool to investigate the activities of cyclic adenosine diphosphate ribose.