Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics
Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics
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DOI：
10.1016/j.ijpharm.2013.06.020
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发表时间：
2013-09-10
影响因子：
5.8
通讯作者：
Moscatelli, Davide
中科院分区：
文献类型：
--
作者：
Ferrari, Raffaele;Colombo, Claudio;Moscatelli, Davide
开发了一种基于聚合物纳米颗粒（NPs）的递送系统，并在乳腺癌治疗的相关生物条件下进行了测试。ε-己内酯（CL）和聚乙二醇（PEG）共聚物已用于一锅法合成不含表面活性剂的PEG化NP，所述NP是单分散的、在生理条件下稳定的并且具有90-250 nm范围内的尺寸。在细胞培养基中研究了这些纳米颗粒的降解行为，并建立了降解时间与起始CL基材料分子量之间的关系。这允许生产具有受控降解动力学的NP。最后，已在4 T1乳腺癌细胞中测试了选定的NP，以检查其毒性并研究其摄取过程，以验证其作为乳腺癌治疗靶向载体的用途。(C)2013爱思唯尔有限公司版权所有。
A delivery system based on polymer nanoparticles (NPs) is developed and tested in relevant biological conditions for breast cancer treatment. epsilon-Caprolactone (CL) and polyethylene glycol (PEG) copolymers have been used for the one pot synthesis of surfactant free PEGylated NPs which are monodispersed, stable in physiological conditions and have size in the range 90-250 nm. The degradation behavior of these NPs has been investigated in cell medium and a relation between degradation time and molecular weight of the starting CL-based material has been established. This allows producing NPs with controlled degradation kinetics. Finally, selected NPs have been tested in 4T1 breast cancer cells to check their toxicity and to investigate the uptake process, in order to validate their use as targeted vectors for breast cancer treatment. (C) 2013 Elsevier B.V. All rights reserved.