Erichrome stain for myelin on osmicated tissue embedded in glycol methacrylate plastic
Erichrome stain for myelin on osmicated tissue embedded in glycol methacrylate plastic
复制标题
嵌在甲基丙烯酸乙二醇酯塑料中的锇组织上的髓鞘质的 Erichrome 染色
DOI：
10.1179/his.1989.12.1.35
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发表时间：
1989
影响因子：
1.1
通讯作者：
Moses Rodriguez
中科院分区：
文献类型：
--
作者：
M. L. Pierce;Moses Rodriguez
摘要本文介绍了改良的Lieb染色技术，用于染色包埋在乙二醇甲基丙烯酸酯塑料中的髓鞘。该技术允许在中枢神经系统中的有髓鞘和脱髓鞘轴突的良好形态细节和持续感染脱髓鞘泰勒病毒株的小鼠脊髓中的病理异常的表征。它是一种简单、快速的技术，可以一次包埋、切片和染色25-30个脊髓横截面。(The J Histotechnol 12：35，1989）。
Abstract This paper describes the modification of the Lieb erichrome technique for staining myelin sheaths' embedded in glycol methacrylate plastic. The technique permits excellent morphologic detail of myelinated and demyelinated axons in the central nervous system and the characterization of pathologic abnormalities in the spinal cord of mice persistently infected with a demyelinating strain of Theiler's virus. It is a simple, rapid technique that allows 25–30 spinal cord cross-sections to be embedded, sectioned and stained at one time. (The J Histotechnol 12:35, 1989).