Moderate intensity, exercise-induced catecholamine release in the preoptic area and anterior hypothalamus in rats is enhanced in a warm environment
Moderate intensity, exercise-induced catecholamine release in the preoptic area and anterior hypothalamus in rats is enhanced in a warm environment
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在温暖的环境中，中等强度的运动引起的大鼠视前区和下丘脑前部的儿茶酚胺释放增强
DOI：
10.1016/j.jtherbio.2017.11.003
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发表时间：
2018
影响因子：
2.7
通讯作者：
Hasegawa Hiroshi
中科院分区：
文献类型：
--
作者：
Zheng Xinyan;Takatsu Satomi;Ishikawa Ryo;Hasegawa Hiroshi
运动过程中的热效应器反应和核心体温（T核心）稳态受环境温度和运动强度的影响。我们以前曾报道，T核心，热损失的反应，并在视前区和下丘脑前部（PO/AH）增加递增跑步机运行过程中的儿茶酚胺释放。然而，没有以前的研究已经检查是否在温暖的环境温度下的体温调节反应的变化与大鼠在中等强度运动过程中的儿茶酚胺反应。因此，本研究的目的是调查的PO/AH中的神经传递的反应，中等强度的运动在不同的环境温度下，并将其与温度调节的变化。采用在体微透析、生物遥测和动物O2/CO2代谢测定系统等方法，同时测定运动大鼠PO/AH单胺水平和体温调节反应。在实验当天，大鼠在23° C或30° C的环境温度下，以18 m min-1的速度在跑步机上以5%的梯度跑步60 min。监测T核心、尾部皮肤温度（T尾;热损失的指标）和耗氧量（V今O2：产热的指标）。多巴胺（DA），去甲肾上腺素（NA），和5-羟色胺（5-HT）的水平测定通过高效液相色谱法（HPLC）与电化学检测。运动使PO/AH的T核、T尾和V ~ O2值以及DA和NA释放量在两种温度下均显著增加，且在温暖环境温度下增加更为明显。结果表明，在T核心，产热和热损失的反应，即使在中等强度运行在温暖的环境中的增加可能与DA和NA释放的PO/AH区域的增加。
Thermoeffector responses and core body temperature (T core) homeostasis during exercise are affected by both ambient temperature and exercise intensity. We have previously reported that T core, heat loss responses, and catecholamine release in the preoptic area and anterior hypothalamus (PO/AH) increased during incremental treadmill running. However, no previous study has examined whether changes in the thermoregulatory responses at warm ambient temperature are related to catecholamine responses during moderate intensity exercise in rats. Therefore, the aim of the present study was to investigate the responsiveness of neurotransmission in the PO/AH to moderate intensity exercise at different ambient temperatures, and to relate this to changes in thermoregulation. We measured the monoamine levels in the PO/AH and the thermoregulatory responses in exercising rats simultaneously using a combination of methods, including in vivo microdialysis, biotelemetry, and animal O 2/CO 2 metabolism measuring system. On the day of experiments, rats ran for 60 min at a speed of 18 m min− 1 on a treadmill at a 5% gradient, in an ambient temperature of 23° C or 30° C. T core, tail skin temperature (T tail; an index of heat loss), and oxygen consumption (V ̇ O 2: an index of heat production) were monitored. Dopamine (DA), noradrenaline (NA), and serotonin (5-HT) levels were measured by high performance liquid chromatography (HPLC) with electrochemical detection. Exercise significantly increased the T core, T tail, and V ̇ O 2 values, as well as DA and NA release in the PO/AH at both temperatures, and the increases were more pronounced at the warm ambient temperature. The results suggest that the increase in the T core, heat production, and heat loss responses even during moderate intensity running in a warm environment are likely associated with an increase in DA and NA release in the PO/AH region.