A role for Phospholipase D in Drosophila embryonic cellularization.
A role for Phospholipase D in Drosophila embryonic cellularization.
复制标题
DOI：
10.1186/1471-213x-6-60
复制
发表时间：
2006-12-07
影响因子：
--
通讯作者：
Frohman MA
中科院分区：
文献类型：
--
作者：
LaLonde M;Janssens H;Yun S;Crosby J;Redina O;Olive V;Altshuller YM;Choi SY;Du G;Gergen JP;Frohman MA
果蝇胚胎的细胞化是一种异常同步的细胞质分裂形式，其中极化膜的延伸部分是通过顶端易位的高尔基衍生囊泡融合而形成的新膜进行的。我们在这里描述了信号酶磷脂酶D （Pld）参与调节这一发育步骤。利用基因靶向的功能分析显示，Pld的缺失阻碍了细胞化，经常导致早期胚胎发育停滞。从机制上讲，慢性Pld缺乏导致高尔基结构异常和分泌囊泡运输。我们的研究结果表明，Pld在细胞化过程中促进高尔基衍生融合能力囊泡的运输。
Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles. We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking. Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.