Cadherin expression is required for the spread of Shigella flexneri between epithelial cells
Cadherin expression is required for the spread of Shigella flexneri between epithelial cells
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福氏志贺氏菌在上皮细胞之间的传播需要钙粘蛋白表达
DOI：
10.1016/0092-8674(94)90358-1
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发表时间：
1994
期刊：
影响因子：
64.5
通讯作者：
R. Mège
中科院分区：
文献类型：
--
作者：
P. Sansonetti;J. Mounier;M. Prévost;R. Mège
福氏志贺氏菌是一种革兰氏阴性病原体，可侵入人类结肠上皮。细菌进入上皮细胞后，逃逸到细胞质中，在细胞内移动，并在细胞间传递。细菌转移肌动蛋白和相关的肌动蛋白结合蛋白，产生一个基于细胞骨架的马达，推动细菌前进。当移动的细菌到达宿主细胞质膜的内表面时，形成一个突起，允许细菌进入邻近的细胞。我们在这里展示了中间连接的成分被细菌用来允许这种通过。使用不产生细胞粘附分子（CAM）的小鼠纤维母细胞肉瘤细胞系S180和分别转染L-CAM和n -钙粘蛋白cDNA的同一细胞系S18OL和S18OcadN，我们证明了钙粘蛋白的表达是发生细胞间扩散所必需的。福氏志贺氏杆菌是一种革兰氏阴性肠道杆菌，通过侵入结肠上皮细胞而引起人类细菌性痢疾（LaBrec等人，1984年），引起强烈的炎症反应，以破坏上皮内膜的脓肿和溃疡为特征。为了了解上皮细胞侵袭的分子和细胞基础，多种哺乳动物上皮细胞系被志贺氏菌感染（Hale等，1979；Mounier等，1992；Vasselon等，1992）。进入宿主细胞是一个细菌导向的吞噬过程（Clerc和Sansonetti， 1987），主要发生在细胞的基底侧膜上（Mounier等，1992）。
Shigella flexnerl, a gram-negative pathogen, invades the human colonic epithelium. After entering epithelial cells, bacteria escape into the cytoplasm, move intracellularly, and pass from cell to cell. The bacterium diverts actin and associated actin-binding proteins to generate a cytoskeleton-based motor that pushes forward the bacterium. As the moving bacterium reaches the inner face of the host-cell cytoplasmic membrane, a protrusion forms that allows passage of this bacterium into a neighboring cell. We show here that components of the intermediate junction are used by the bacterium to allow this passage. Using S180, a mouse fibroblastic sarcoma cell line that does not produce cell adhesion molecules(CAM), and S18OL and S18OcadN, the same cell line transfected with L-CAM and N-cadherin cDNA, respectively, we demonstrate that expression of a cadherin is required for cell-to-cell spread to occur.Shigella flexneri, a gram-negative enteric bacillus, causes bacillary dysentery in humans by invading epithelial cells of the colon (LaBrec et al., 1984) where it elicits an intense inflammatory reaction, characterized by abscesses and ulcerations that destroy the epithelial lining. To understand the molecular and cellular bases of epithelial cell invasion, various mammalian epithelial cell lines have been infected by Shigella (Hale et al., 1979; Mounier et al., 1992; Vasselon et al., 1992). Entry into host cells is a process of bacterium-directed phagocytosis (Clerc and Sansonetti, 1987) which occursessentiallyon the basolatera1 membrane of cells (Mounier et al., 1992).
DOI：
10.1073/pnas.85.19.7274
发表时间：
1988-10-01
影响因子：
11.1
作者：
MEGE, RM;MATSUZAKI, F;EDELMAN, GM
通讯作者：
EDELMAN, GM