Characterization of the pufferfish Takifugu rubripes apolipoprotein multigene family
Characterization of the pufferfish Takifugu rubripes apolipoprotein multigene family
复制标题
DOI：
10.1016/j.gene.2004.11.015
复制
发表时间：
2005-02-14
期刊：
影响因子：
3.5
通讯作者：
Watabe, S
中科院分区：
文献类型：
--
作者：
Kondo, H;Morinaga, K;Watabe, S
我们的特点是载脂蛋白多基因家族的河豚红鳍东方鲀。河豚主要含有28-kDa，27-kDa，和14-kDa的载脂蛋白在其血浆中，并分别命名为载脂蛋白-28 kDa，载脂蛋白-27 kDa，和载脂蛋白-14 kDa。N-末端氨基酸序列分析表明，河豚apo-28 kDa和apo-27 kDa的氨基酸序列完全相同，但前者有一个额外的前肽，两者都是apoA-I的同源物。河豚鱼apo-14 kDa的序列与先前报道的鳗鱼apo-14 kDa的序列同源，两者显然都是鱼类特有的。在计算机筛选中，使用公开可用的河豚基因组数据库确认了河豚apoA-I和apo-14 kDa基因。该数据库还包含编码四种apoA-IV、一种apoC-II和两种apoE的基因。因此，河豚鱼含有9个编码载脂蛋白多基因家族的基因。两个apoA-IV和一个apoE基因串联排列并位于一个支架上。因此，这两组基因形成了两个基因簇。apoC-II和apo-14 kDa基因也位于单个支架上。apoA-I和apo-14 kDa基因转录本主要在肝脏中表达，在脑中表达较少。前一基因的转录本在肠道中也有表达。与此相反，编码四个apoA-IV，一个apoC-II和两个apoE的转录本主要在肠道中表达。这些河豚载脂蛋白的结构细节及其基因转录本的组织分布为更好地理解载脂蛋白多基因家族的进化关系提供了新的证据。(c)2004 Elsevier B. V.保留所有权利。
We have characterized the apolipoprotein multigene family of the pufferfish Takifugu rubripes. The pufferfish mainly contains 28-kDa, 27-kDa, and 14-kDa apolipoproteins in its plasma and was designated apo-28 kDa, apo-27 kDa, and apo-14 kDa, respectively. N-terminal amino acid sequencing revealed that pufferfish apo-28 kDa and apo-27 kDa have an identical amino acid sequence except an additional propeptide in the former; and both are homologues of apoA-I from other animals. The sequence of pufferfish apo-14 kDa is homologous to that of eel apo-14 kDa previously reported, both being apparently specific to fish. In silico screening, using the publicly available Fugu genome database confirmed the pufferfish apoA-I and apo-14 kDa genes. The database further contained the genes encoding four types of apoA-IV, one apoC-II and two types of apoE. Thus, pufferfish contains nine genes encoding apolipoprotein multigene family. Two apoA-IV and one apoE genes were tandemly arrayed and located on one scaffold. Thus two sets of these genes formed two gene clusters. The apoC-II and apo-14 kDa genes are also located on a single scaffold. apoA-I and apo-14 kDa gene transcripts were mainly expressed in liver and less abundantly in brain. The transcripts of the former gene were also observed in intestine. In contrast, the transcripts encoding four apoA-IVs, one apoC-II, and two apoEs were mainly expressed in intestine. These structural details of pufferfish apolipoproteins and tissue distribution of their gene transcripts provide a novel evidence for better understanding of evolutionary relationships of apolipoprotein multigene family. (c) 2004 Elsevier B.V. All rights reserved.