In vitro antibiofilm activity of bioactive glass S53P4
In vitro antibiofilm activity of bioactive glass S53P4
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DOI：
10.2217/fmb.14.20
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发表时间：
2014-05-01
影响因子：
3.1
通讯作者：
Romano, Carlo Luca
中科院分区：
文献类型：
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作者：
Drago, Lorenzo;Vassena, Christian;Romano, Carlo Luca
目的：研究不同配方的生物活性玻璃(BAG)-S53P4对耐甲氧西林金黄色葡萄球菌和多重耐药铜绿假单胞菌在假体材料上产生的细菌生物膜的干扰能力。材料与方法：通过晶体紫和共聚焦激光扫描显微镜两种不同的分析方法，在不同的时间点评估三种配方的生物玻璃的抗生物膜活性。结果：bag - s53p4处理后的样品与对照样品的整个生物膜有显著差异，而不同配方的抗生物膜活性无明显变化。共聚焦激光扫描显微镜分析证实，BAG-S53P4处理后，金黄色葡萄球菌和铜绿假单胞菌生物膜的生物量和总细胞体积均显著减少。结论：BAG-S53P4可作为治疗假体生物膜相关感染的优良佐剂。
Aim: This work aimed to investigate the ability of different formulations of bioactive glass (BAG)-S53P4 to interfere with bacterial biofilm produced on prosthetic material by methicillin-resistant Staphylococcus aureus and multi-drug-resistant Pseudomonas aeruginosa. Materials & methods: Antibiofilm activity of three formulations of bioglass was assessed at different time points through two different analyses: Crystal Violet and confocal laser scanning microscopy assays. Results: Significant differences in the whole biofilm were observed between BAG-S53P4-treated and control samples, while no marked changes in antibiofilm activity were observed among the tested formulations. Data from colorimetric assay were confirmed by confocal laser scanning microscopy analysis, which evidenced the significant reduction in biomass and a decrease of total cell volume when both S. aureus and P. aeruginosa biofilms were treated with BAG-S53P4. Conclusion: BAG-S53P4 can be considered as an excellent adjuvant in the treatment of prosthetic infections related to biofilm.