Intrafibrillar Mineralized Collagen-Hydroxyapatite-Based Scaffolds for Bone Regeneration.
Intrafibrillar Mineralized Collagen-Hydroxyapatite-Based Scaffolds for Bone Regeneration.
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DOI：
10.1021/acsami.0c00275
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发表时间：
2020-03
影响因子：
9.5
通讯作者：
Le Yu;D. Rowe;I. Perera;Jiyao Zhang;S. Suib;X. Xin;M. Wei
中科院分区：
文献类型：
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作者：
Le Yu;D. Rowe;I. Perera;Jiyao Zhang;S. Suib;X. Xin;M. Wei
作为组织工程领域的主要挑战之一，大面积骨缺损已引起研究者的广泛关注。胶原（Collagen，Col）和羟基磷灰石（Hydroxyapatite，HA）是天然骨中含量最丰富的蛋白质和主要成分，因其良好的生物相容性和生物可降解性，常被用作组织工程中的仿生复合材料。在这项研究中，新的纤维内矿化Col-HA为基础的支架，无论是在细胞或层状微结构构建，通过仿生方法建立，以提高新的骨再生能力的组织工程支架。此外，铁（Fe）和锰（Mn），两个人体必需的微量元素，成功地纳入到层状支架，以进一步提高这些生物材料的骨诱导性。结果发现，层状支架表现出更好的成骨能力相比，在内部和商业Col-HA为基础的细胞支架在体外和体内。同时，Fe/Mn掺入进一步放大了层状支架的成骨促进作用。更重要的是，观察到的协同效应，在铁和锰双元素纳入层状支架的骨髓间充质干细胞（BMSCs）的体外成骨分化和体内骨再生加载新鲜骨髓细胞。本研究为骨再生功能性支架材料的制备提供了一种简单实用的方法。
As one of the major challenges in the field of tissue engineering, large skeletal defects have attracted wide attention of researchers. Collagen (Col) and hydroxyapatite (HA), the most abundant protein and main component in natural bone, are usually used as a biomimetic composite material in tissue engineering due to its excellent biocompatibility and biodegradability. In this study, novel intrafibrillar mineralized Col-HA-based scaffolds, constructed in either cellular or lamellar microstructures, were established through a biomimetic method to enhance the new bone regenerating capability of tissue engineering scaffolds. Moreover, iron (Fe) and manganese (Mn), two essential trace elements in body, were successfully incorporated into the lamellar scaffold to further improve the osteoinductivity of these biomaterials. It was found that the lamellar scaffolds demonstrated better osteogenic abilities compared to both in-house and commercial Col-HA-based cellular scaffolds in vitro and in vivo. Meanwhile, Fe/Mn incorporation further amplified the osteogenic promotion of the lamellar scaffolds. More importantly, a synergistic effect was observed in the Fe and Mn dual elements incorporated lamellar scaffolds for both in vitro osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and in vivo bone regeneration loaded with fresh bone marrow cells. This study provides a simple but practical strategy for creation of functional scaffolds for bone regeneration.