Heterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture
Heterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture
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DOI：
10.1016/s0032-9592(96)00006-4
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发表时间：
1996-01-01
影响因子：
4.4
通讯作者：
Johns, MR
中科院分区：
文献类型：
--
作者：
Chen, F;Johns, MR
研究了使用异养恒化器培养通过避免底物醋酸盐对生长的抑制来获得相对较高的莱茵衣藻细胞密度的可能性。与分批培养相比，连续培养显着提高了细胞干重浓度和生产力。据推测，这是由于连续培养中乙酸盐浓度低所致。在进料乙酸盐浓度为3.4 g/L时，获得的最高细胞浓度为1.5 g/L。在乙酸盐浓度达到该水平时，修改的Monod模型成功地预测了稳态细胞浓度和细胞生产率。然而，尽管使用了更高的进料乙酸盐浓度（即5.1 g/升），但没有实现细胞浓度的进一步增加，其中稳态乙酸盐浓度低于抑制水平（即0.4 g/升）。这种抑制作用可能是由于钠，而不是乙酸本身的文化。
The possibility of using heterotrophic chemostat culture to achieve relatively high cell densities of Chlamydomonas reinhardtii by avoiding the inhibition of growth by the substrate, acetate was investigated Continuous culture gave significant improvement in cell dry weight concentration and productivity compared to batch culture. It is postulated that this was due to low acetate concentration in the continuous culture. The highest cell concentration obtained was 1.5 g/litre at a feed acetate concentration of 3.4 g/litre. At acetate concentrations up to this level, the modified Monod model successfully predicted the steady-state cell concentration and cellular productivity. However no further increase in cell concentration was achieved, despite the use of higher feed acetate concentrations (i.e. 5.1 g/litre), in which, steady-state acetate concentrations were below the inhibition level (i.e. 0.4 g/litre). This inhibitory effect was probably due to sodium rather than to acetate per se in the cultures.