Variable-sized free episomes of Shope papilloma virus DNA are present in all non-virus-producing neoplasms and integrated episomes are detected in some.
Variable-sized free episomes of Shope papilloma virus DNA are present in all non-virus-producing neoplasms and integrated episomes are detected in some.
复制标题
所有不产生病毒的肿瘤中均存在大小不同的肖普乳头状瘤病毒 DNA 游离附加体，并且在某些肿瘤中检测到整合的附加体。
DOI：
10.1073/pnas.79.3.790
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发表时间：
1982
影响因子：
11.1
通讯作者：
Stevens,JG
中科院分区：
文献类型：
--
作者：
Wettstein,FO;Stevens,JG
本文研究了兔乳头状瘤病毒DNA在病毒诱发的家兔非产生性肿瘤中的状态。通过一维琼脂糖凝胶电泳将病毒特异性序列解析成许多不同的条带。二维凝胶电泳，氯化铯/碘化丙啶密度平衡离心，部分消化与限制性内切酶，和S1核酸酶消化允许我们确定的频带作为免费的病毒游离体代表的环状分子的大小增加。在某些肿瘤（乳头状瘤和癌）中，高达25%的病毒特异性DNA是线性的，并与细胞DNA共迁移。在用EcoRI和Sal I（将Shope DNA切割一次的酶）消化DNA后，通过检测一个肿瘤中的病毒-细胞连接带，提示至少部分这些序列的整合。最后，发现乳头状瘤和癌中病毒DNA的物理状态是相似的，尽管癌中游离的附加体通常较大。
The state of rabbit (Shope) papilloma virus DNA in virus-induced nonproducing tumors on domestic rabbits was investigated. Virus-specific sequences were resolved into many distinct bands by one-dimensional agarose gel electrophoresis. Two-dimensional gel electrophoresis, CsCl/propidium iodide density equilibrium centrifugation, partial digestion with a restriction endonuclease, and S1 nuclease digestion permitted us to identify the bands as free viral episomes representing circular molecules of increasing size. In some tumors (both papillomas and carcinomas), up to 25% of the virus-specific DNA was linear and comigrated with cellular DNA. Integration of at least some of these sequences was suggested by the detection of viral-cellular junction bands in one tumor after digestion of DNA with EcoRI and Sal I, enzymes that cut Shope DNA once. Finally, the physical states of viral DNA in papillomas and carcinomas were found to be similar, although free episomes were generally larger in carcinomas.
自然发生的癌症中的病毒
DOI：
--
发表时间：
1980
期刊：
影响因子：
--
作者：
M. Essex;G. Todaro;H. Hausen
通讯作者：
H. Hausen