Chemical characterization and gastroprotective effect of an isolated polysaccharide fraction from Bletilla striata against ethanol-induced acute gastric ulcer
Chemical characterization and gastroprotective effect of an isolated polysaccharide fraction from Bletilla striata against ethanol-induced acute gastric ulcer
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白芨分离多糖组分的化学特性和胃保护作用对乙醇诱导的急性胃溃疡
DOI：
10.1016/j.fct.2019.05.047
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发表时间：
2019-09-01
影响因子：
4.3
通讯作者：
Zhang, Jinming
中科院分区：
文献类型：
--
作者：
Zhang, Chen;Gao, Fei;Zhang, Jinming
采用超滤膜法提取白芨多糖，研究其化学性质及对大鼠乙醇性胃溃疡的保护作用。该BSP组分由甘露糖和葡萄糖组成，摩尔比约为2.4：1，分子量为146 KDa。FT-IR、NMR和XRD分析表明，BSP组分中含有α-Man和β-Glc残基，结晶度较低。该多糖具有明显的ABTS和FRAP清除活性，对人胃上皮GES-1细胞无毒性。100 mg/kg BSP连续3 d灌胃可显著预防乙醇所致胃粘膜损伤的形成。它还可以降低促炎细胞因子的水平，包括TNF-α，IL-1 β，IL-6和IL-18，以及胃组织中的MPO活性。此外，BSP组分还具有抗氧化活性，增加胃组织中作为防御因子的PEG（2）的含量，并抑制MAPK/NF-κ B信号通路。上述结果表明，BSP组分的胃保护作用可能与降低促炎细胞因子和氧化应激以及抑制MAPK/NF-κ B通路有关。我们的研究结果为BSP可能成为一种很有前途的预防胃溃疡的植物药提供了有力的证据。
The chemical characterization and protective role against ethanol-induced gastric ulcerated rats of a polysaccharide fraction from Bletilla striata (BSP) collected by ultrafiltration membrane approach were evaluated. This BSP faction was consisted of mannose and glucose at a molar ratio of 2.4:1 approximately, with a molecular weight of 146 KDa. FT-IR, NMR and XRD spectra indicated that BSP faction contained alpha-Man and beta-Glc residues with low overall crystallinity. The polysaccharide exhibited significant scavenging activities of ABTS and FRAP, as well as non-toxicity against human gastric epithelial GES-1 cells. Oral administration with 100 mg/kg of BSP for 3 days continuously could significantly prevent the formation of ethanol-induced gastric mucosal lesion. It could also reduce the levels of pro-inflammatory cytokines, including TNF-alpha, IL-1 beta, IL-6, and IL-18, and MPO activity in gastric tissue. Additionally, the BSP faction exhibited antioxidant activity, increased the content of PEG(2) as a defensive factor, and suppressed MAPK/NF-kappa B signaling pathway in gastric tissue. These results indicated that the gastroprotective activity of BSP faction could be attributed to the reduction of pro-inflammatory cytokines and oxidative stress and the inhibition of MAPK/NF-kappa B pathways. Our results provided substantial evidence that BSP could be a promising phytomedicine for gastric ulcer prevention.