Fucoxanthin and fucoxanthinol enhance the amount of docosahexaenoic acid in the liver of KKAy obese/diabetic mice.
Fucoxanthin and fucoxanthinol enhance the amount of docosahexaenoic acid in the liver of KKAy obese/diabetic mice.
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DOI：
10.1021/jf070110q
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发表时间：
2007-05
影响因子：
6.1
通讯作者：
Takayuki Tsukui;Kensuke Konno;M. Hosokawa;Hayato Maeda;Tokutake Sashima;K. Miyashita
中科院分区：
文献类型：
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作者：
Takayuki Tsukui;Kensuke Konno;M. Hosokawa;Hayato Maeda;Tokutake Sashima;K. Miyashita
本研究检测了膳食岩藻黄素或岩藻黄醇对肥胖/II型糖尿病模型KKAy小鼠肝脏中二十二碳六烯酸（DHA）含量的影响。在第一个实验中，给小鼠喂食含有粗岩藻黄质或甘油糖脂的饮食4周。结果显示，喂食0.53%粗岩藻黄质的小鼠的DHA水平显著增加，从对照小鼠的2.3%增加到总肝脏脂质的脂肪酸组成的5.1%。另一方面，在喂食粗甘油糖脂的小鼠中，DHA水平占总肝脏脂肪酸的比例保持不变。为了阐明肝脏DHA的增强，在第二个实验中，KKAy小鼠喂食含有纯化的岩藻黄质或其脱乙酰衍生物岩藻黄醇的饮食。使用内标进行定量分析的结果显示，在喂食0.2%岩藻黄质的小鼠中，肝脏DHA的量是对照小鼠的2倍，而小肠中的DHA水平保持不变。此外，与对照小鼠相比，0.2%岩藻黄醇分别导致肝脏DHA和花生四烯酸的量增加1.8倍和1.2倍。这些结果首次表明，膳食岩藻黄质和岩藻黄醇提高了KKAy小鼠肝脏中DHA的量。
This study examined the effect of dietary fucoxanthin or fucoxanthinol on the amount of docosahexaenoic acid (DHA) in the liver of KKAy mice, a model for obese/type II diabetes. In the first experiment, mice were fed diets containing crude fucoxanthin or glyceroglycolipid for 4 weeks. Results showed a significant increase in the level of DHA in mice fed 0.53% crude fucoxanthin, from 2.3% in control mice to 5.1% of fatty acid composition of total liver lipids. On the other hand, in mice fed crude glyceroglycolipid, the level of DHA as a proportion of total liver fatty acids remained unchanged. To clarify the enhancement of hepatic DHA, in the second experiment, KKAy mice were fed a diet containing purified fucoxanthin or its deacetylated derivative, fucoxanthinol. Results from a quantitative analysis using an internal standard showed that in mice fed 0.2% fucoxanthin, the amount of hepatic DHA was 2-fold higher than in control mice, whereas DHA levels in the small intestine remained unchanged. Furthermore, 0.2% fucoxanthinol led to 1.8- and 1.2-fold increases in the amount of hepatic DHA and arachidonic acid compared to control mice, respectively. These results indicate for the first time that dietary fucoxanthin and fucoxanthinol enhance the amount of DHA in the liver of KKAy mice.