New cyclopeptide alkaloids from the whole plant of Justicia procumbens L
New cyclopeptide alkaloids from the whole plant of Justicia procumbens L
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Justicia procumbens L全草中新的环肽生物碱
DOI：
10.1080/14786419.2020.1758090
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发表时间：
2020-04-27
影响因子：
2.2
通讯作者：
Jin, Hong
中科院分区：
文献类型：
--
作者：
Lv, Jin-Peng;Yang, Shu;Jin, Hong
摘要从平卧Justicia procumbens L.全草乙醇提取物中分离得到3个新的14元环肽生物碱，分别命名为justicianenes B-D（1-3），根据详细的NMR谱数据确定了它们的结构，并通过ECD谱确定了环肽生物碱中成环α-氨基酸的绝对立体化学。评价了分离的化合物对人癌细胞系（包括乳腺癌MCF-7、宫颈癌HeLa、肺癌A549和H460）的体外细胞毒性，并且diphyllin（14）显示出对HeLa、A549和H460细胞的中等细胞毒性，IC 50分别为9.13、23.12、42.34 μM，justicianene D对MCF-7细胞的细胞毒性较弱，在90 µM浓度下抑制率为50%。图形摘要
Abstract Three novel 14-membered cyclopeptide alkaloids, justicianenes B-D (1-3), were isolated from the EtOH extract of the whole plant of Justicia procumbens L., and their structures were determined on the basis of detailed NMR spectroscopic data and the absolute stereochemistry of the ring-bonded α-amino acids in the cyclopeptide alkaloids were determined by ECD spectra. The isolated compounds were evaluated for their in vitro cytotoxicity against human cancer cell lines, including brest cancer MCF-7, cervix carcinoma HeLa, lung cancer A549 and H460, and diphyllin (14) showed moderate cytotoxicity against the HeLa, A549 and H460 cells with IC50 of 9.13, 23.12, 42.34 µM, respectively, justicianene D showed weak cytotoxicity against the MCF-7 cell with inhibition rate of 50% at the concentration of 90 µM. Graphical Abstract