Actions of neuropeptide W in paraventricular hypothalamus: implications for the control of stress hormone secretion
Actions of neuropeptide W in paraventricular hypothalamus: implications for the control of stress hormone secretion
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DOI：
10.1152/ajpregu.00396.2004
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Samson, WK
中科院分区：
文献类型：
--
作者：
Taylor, MM;Yuill, EA;Samson, WK
神经肽 W (NPW) 在位于下丘脑和脑干的神经元中产生，其受体存在于下丘脑中，特别是室旁核 (PVN) 中。脑室内（ICV）给予 NPW，以剂量相关的方式激活下丘脑-垂体-肾上腺轴（根据清醒大鼠的血浆皮质酮水平确定），但在相同剂量下，不会刺激催产素或加压素释放到外周循环中，也不会改变血压或心率。在有意识的雄性大鼠中，ICV 给药的 NPW 刺激下丘脑-垂体-肾上腺轴的能力被促肾上腺皮质激素释放激素拮抗剂静脉内预处理所阻断。这表明 NPW 在 PVN 的小细胞分裂中发挥作用。事实上，在下丘脑切片制剂（全细胞贴片记录）中，NPW 的浴应用可以去极化并增加大多数电生理学鉴定的推定神经内分泌 PVN 神经元的尖峰频率。在 TTX 存在的情况下，对膜电位的影响仍然存在，表明它们是对这些神经内分泌细胞的直接突触后作用。我们的数据表明，大脑中产生的内源性 NPW 可能在神经内分泌应激反应中发挥生理相关作用。
Neuropeptide W (NPW) is produced in neurons located in hypothalamus and brain stem, and its receptors are present in the hypothalamus, in particular in the paraventricular nucleus (PVN). Intracerebroventricular (ICV) administration of NPW activated, in a dose-related fashion, the hypothalamic-pituitary-adrenal axis, as determined by plasma corticosterone levels in conscious rats but, at those same doses, did not stimulate the release of oxytocin or vasopressin into the peripheral circulation or alter blood pressure or heart rate. The ability of ICV-administered NPW to stimulate the hypothalamic-pituitary-adrenal axis in conscious male rats was blocked by intravenous pretreatment with a corticotropin-releasing hormone antagonist. This suggested an action of NPW in the parvocellular division of the PVN. Indeed, in hypothalamic slice preparations (whole cell patch recording), bath application of NPW depolarized and increased the spike frequency of the majority of electrophysiologically identified putative neuroendocrine PVN neurons. Effects on membrane potential were maintained in the presence of TTX, suggesting them to be direct postsynaptic actions on these neuroendocrine cells. Our data suggest that endogenous NPW, produced in brain, may play a physiologically relevant role in the neuroendocrine response to stress.